乳腺导管内增生性病变中ER、Ki-67和cyclin D1的表达被引量：6

Expression of ER,Ki-67 and cylin D1 in introductal proliferative lesions of breast

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摘要目的探讨ER、Ki-67和cyclin D1在乳腺导管内增生性病变中的表达及意义。方法采用免疫组化和免疫荧光双标记法对56例乳腺导管内增生性病变进行ER、Ki-67和cyclin D1染色标记。结果正常乳腺组织中仅有散在的少数上皮细胞呈ER阳性表达。在普通型导管增生(usual ductal hyperplasia,UDH)中ER表达比正常乳腺组织增加,但ER阳性细胞呈不连续分布,阳性细胞间有较多的阴性细胞。非典型性导管增生(atypical ductal hyperplasia,ADH)和低级别原位导管癌(ductal carci-noma in situ,DCIS)中ER表达比UDH明显增加(P<0·05),ER阳性细胞呈连续的片状分布,阳性细胞间较少或没有ER阴性细胞。ADH和低级别DCIS中ER表达较高级DCIS显著(P<0·01)。DCIS中Ki-67和cyclin D1表达高于UDH(P<0·05),并与UDH、ADH和DCIS的组织学分组呈正相关(r=0·352,P<0·05和r=0·390,P<0·05)。正常乳腺组织中上皮细胞内无ER和Ki-67同时表达。在UDH中有极少数上皮细胞ER和Ki-67同时表达,而在ADH和DCIS中ER和Ki-67同时表达的细胞明显增加。结论从正常乳腺组织到UDH、ADH、低级DCIS的恶性转化过程中伴有ER表达的逐渐增高。ER过度表达及ER和Ki-67在上皮细胞内同时表达可能是某些乳腺癌发生过程中的早期事件。 Purpose To investigate the expression and association of ER, Ki-67 and cyclin D1 in usual ductal hyperplasia （UDH） , atypical ductal hyperplasia（ADH） and ductal carcinoma in situ （DCIS） in the breast. Methods Immunohistochemistry was used to determine the expression of ER, Ki-67 and cyclin D1 and double-lablling immunofluorescence technique was used to observe the coexpression of ER and Ki-67 in 56 cases of introductal proliferative lesions. Results The expression and distribution of ER-positive cells were significantly different in UDH, ADH and DCIS. The ER-positive cells were much more in UDH than in normal terminal ductal lobular units. The distribution of ER-positive cells interspersed amid ER-negative cells within UDH. However, the ER positive cells showed marked increases in ADH and low-grade DCIS（ P 〈0. 05） , and distributed among almost all constituent cells. The expression of Ki-67 and cyclin D1 was significant difference between UDH and DCIS（ P 〈 0. 05 ） , and a positive correlation was found between Ki- 67 and morphological classification of intraductal proliferative lesions （ r = 0. 352, P 〈0. 05 ） as well as cyclin D1 （ r = 0. 390, P 〈 0. 05 ）. Double-labelling immunofluorescence showed that there was no coexpression of ER and Ki-67 in normal breast tissue. The coexpression of the two markers was found in ADH and increased in DCIS. Condusions Overexpression of ER, Ki-67 and cyclin D1 is significantly associated with the transformation of normal cells and UDH into ADH and DCIS. The coexpression of ER and Ki-67 may present the early change in carcinogenesis of breast cancer.

作者周成军张庆慧张廷国李红王妍刘志艳

机构地区山东大学齐鲁医院病理科

出处《临床与实验病理学杂志》 CAS CSCD 北大核心 2006年第4期454-457,共4页 Chinese Journal of Clinical and Experimental Pathology

关键词乳腺肿瘤导管内增生性病变雌激素受体 KI-67 细胞周期蛋白D1 breast neoplasms intraductal proliferative lesions estrogen receptors Ki-67 cyclin D1

分类号 R737.9 [医药卫生—肿瘤] R341.31 [医药卫生—基础医学]

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