摘要
目的:建立白术内酯Ⅲ的反相高效液相色谱分析方法,并对其在大鼠体内的药代动力学和组织分布特性进行分析研究。方法:生物样品采用液-液萃取法提取,用Hypersil C18ODS色谱柱(150 mm×4.6 mm,5μm),柱温25℃,甲醇-水(67∶33)为流动相,流速1.0 m l/m in,检测波长为220 nm。结果:方法的平均回收率为85.12%(RSD=5.57%),最低检测限为0.10μg/m l,测定血药浓度线性范围为0.2μg/m l^18.5μg/m l(r=0.9996)。日内、日间精密度(RSD)分别为0.98%~6.19%,12.95%~15.48%;SD大鼠一次性灌胃100μg/m l白术内酯Ⅲ后C-t曲线呈一级吸收二室模型,达峰时间为(0.85±0.01)h。给药1.5 h时,在主要效应器官的浓度分布特点是:C肺>C小脑>C心>C大脑;在主要消除器官的浓度分布特点:C脾>C肝>C肾。结论:本法准确、稳定、灵敏度较高,可用于白术内酯Ⅲ的体内过程研究。白术内酯Ⅲ在大鼠体内吸收较快,消除速度快,在肺部分布最多。
Objectvie： To establish an HPLC mehod for the analysis of pharmacokinetics and tissue distribution of atractylenolide Ⅲ in rats. Methods： The biological samples were extracted with ether. The chromatographic conditions were as follows： Hypersil ODS column（ 150 mm ×4. 6 mm, 51μm） was used. The mobile phase was methnol/warter（67： 33） with a flow rate of 1.0 ml/min under the column temperature of 25℃, and the detection wavelength was set at 220 nm. Results ： The recovery of the method was 85.12% （ RSD = 5.57% ）. The linear range was 0. 2 μg/ml -18.5 μg/ml（ r = 0. 9996 ） in rat plasma. The Lowest Limit of detection was 0. 10μg/ ml （S/N 〉 3 ）. The within-day and between-day precision were from 0. 98% to 6. 19% and 12. 95% to 15.48%, respectively. After oral administration of atractylenolide Ⅲ （100 mg/kg）, the concentration-time profiles of atractylenonlide Ⅲ fit a two compartment model. In main effect tissues, the atractylenolide Ⅲ concentration was followed as in order Clung 〉 Ccerebellum 〉 Cheatt 〉 Ccerebrum, and that was Cspleen 〉 Cliver 〉 Ckidney in eliminated tissues. Conclusion： The method is accurate, stable and reliable, and can be used for the investigation of atractylenolide Ⅲ in plasma and tissues of rats,
出处
《中药材》
CAS
CSCD
北大核心
2006年第8期807-809,共3页
Journal of Chinese Medicinal Materials
