摘要
目的:探讨痰热清注射液对新生大鼠高氧肺损伤的影响。方法:选用生后2天的Wistar大鼠乳鼠90只随机分为高氧组、治疗组、正常组,高氧组与治疗组制备成高氧肺损伤模型,治疗组制备模型后腹腔注射痰热清注射液。各组模型制备后的第3、7、14天时随机分批处死大鼠,取肺组织,行HE染色观察肺的发育程度,并行免疫组织化学染色,对肺组织中转化生长因子β1、转化生长因子βⅠ、Ⅱ型受体行定位、半定量评分,对肺巨核细胞行细胞计数,将各组的测定值进行比较分析。结果:高氧组肺组织中转化生长因子β1、转化生长因子βⅠ、Ⅱ型受体的表达明显强于正常组、痰热清组,有显著性差异(P<0.01)。高氧组的肺巨核细胞计数少于正常组、痰热清组,有显著性差异(P<0.01)。结论:痰热清注射液在新生大鼠高氧肺损伤时对肺组织有保护作用,对支气管—肺发育不良有一定的预防作用。
Objective： To discuss the protect effect of Tan Re - Qing injection on lung injury induced by hyperoxia in newborn rats. Methods： 90 newborn rats were assigned to hyperoxie group, Tan Re - Qing treatment group and control group randomly. They were sacrificed at the day of the 3rd, 7th and 14th after modeled, then their lungs were obtained for HE staining and immunohistochemistry to observe the growth degree and measure the TGF - β1, TGF - βRⅠ, TGF - βRⅡ, and were performed lung megakaryocytes cell count. The results of each group were statistically compared. Results： The expression of TGF - β1, TGF - βRⅠ, TGF - βRⅡ in hyperoxie group was significantly higher than that in Tan Re - Qing treatment group and control group （ P 〈 0. 01 ）, lung megakaryocytes cell count in hyperoxic group was significantly lower than that in control group （ P 〈 0. 01 ） . Conclusion： Treatment with Tan Re - Qing injection on lung injury induced by hyperoxia in newborn rots could play the protective role, and decrease the occurrence of bronehopulmonary dysplasia （BPD） .
出处
《中国妇幼保健》
CAS
北大核心
2006年第16期2266-2268,共3页
Maternal and Child Health Care of China
