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苦参不同器官中的生物碱含量分析 被引量:7

Analysis of alkaloid content in different organs in Sophora flavescens
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摘要 [目的]探讨苦参不同器官中的生物碱含量,为该资源植物的合理应用提供依据.[方法]用薄层色谱法(TLC)分析测定了辽宁产苦参不同器官中苦参碱及氧化苦参碱含量.[结果]各类器官,除叶片外,氧化苦参碱含量均显著超过苦参碱含量;苦参碱含量以种子中的为最高,占干重的0.065%,其次为茎(0.034%)、根(0.016%)、叶(0.013%);氧化苦参碱含量以根最高,占干重的3.847%,其次为种子(1.982%)、茎(0.140%),叶中未检测到;粗老根(直径大于1cm)与细嫩根(直径在0.5-1.0cm)中的两种生物碱含量差别不大.[结论]达到一定生长年限后,生物碱积累不再有明显增加而趋于饱和;大部分器官中,氧化苦参碱含量高于苦参碱含量;种子及茎更适合作为苦参碱的提取器官. [ Objective ] In order to right use Sephora flavescens plant resource, contents of matrine and oxymatrine of various organs of Sophora flavescens from Liaoning province were tested in the present paper. [ Methods] TLC method. [ Results] Matrine of seeds is the highest in content among all the tested organs,being up to 0. 065% of dry weight,then stems (0. 034% ) ,roots (0. 016% ) and leaves (0. 013% ) ;The content of oxymatrine in roots is the highest, up to 3. 847% of dry weight, and then seeds ( 1. 982% ) and stems ( 0. 140% ), no oxymatrine was detected in leaves. The alkaloid content is not significantly different between old rough roots more than 1.0cm in diameter and young thin ones of 0.5 - 1.0 cm in diameter. [ Conclusions ] Contents of alkaloids in Sophora flavescens plants will keep stabilization in mature plants although the plants still grow;Oxymatrine is more than matrine in most organs of Sophora flavescens ; Seeds and stems are appropriate organs for matrine extraction.
出处 《大连大学学报》 2006年第4期79-81,共3页 Journal of Dalian University
关键词 苦参 苦参碱 氧化苦参碱 薄层层析 含量分析 Sophora flavescens matrine oxymatrine TLC content analysis
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