摘要
背景:肠易激综合征(IBS)是一种功能性胃肠道疾病,其病因和发病机制迄今尚未完全阐明。目的:探讨5-羟色胺(5-HT)和5-HT3受体(5-HT3R)在腹泻型IBS(D-IBS)患者结肠黏膜中的变化及其临床意义。方法:对18名正常人和28例D-IBS患者行结肠镜检查并分别取升结肠、横结肠、降结肠和乙状结肠黏膜标本各两块。以免疫组化方法检测5-HT阳性细胞;以蛋白质印迹分析半定量检测5-HT3R的表达。结果:D-IBS组4个部位结肠黏膜的5-HT阳性细胞数和5-HT3R的表达均较正常对照组显著增多(P<0.05),但4个部位之间相互比较均无显著差异。结论:D-IBS患者结肠黏膜5-HT合成和分泌增多,5-HT3R表达上调,提示5-HT和5-HT3R可能为D-IBS的分子生物学基础之一,为D-IBS的治疗提供了分子靶点。
Background： Irritable bowel syndrome （IBS） is a functional gastrointestinal tract disease. Its cause and mechanism remain unclear. Aims： To appraise the changes and clinical significances of 5-hydroxytryptamine （5-HT） and 5-HT3 receptor （5-HT3R） in colonic mucosa of diarrhea-predominant IBS （D-IBS） patients. Methods： The distribution of 5-HT positive cells was measured by immunohistochemistry and expression of 5-HT3R by Western blot in four biopsied sections from the ascending, transverse, descending and sigmoid colon of 18 healthy controls and 28 D-IBS patients. Results： The number of 5-HT positive cells and the expression of 5-HT3R in all sections of colonic mucosa in D-IBS patients increased markedly compared to those in healthy controls （P〈0.05）. No significant differences in expression of 5-HT3R were found among the four colonic sections as well as the number of 5-HT positive cells. Conclusions： The synthesis and excretion of 5-HT and expression of 5-HT3R were increased significantly in D-IBS patients, suggesting that 5-HT and 5-HT3R are involved in the pathogenesis of D-IBS, which may offer a molecular target for treatment.
出处
《胃肠病学》
2006年第8期477-480,共4页
Chinese Journal of Gastroenterology
基金
苏威全国肠易激综合征研究基金资助
关键词
腹泻
肠易激综合征
血清素
受体
血清素
Diarrhea
Irritable Bowel Syndrome
Serotonin
Receptors, Serotonin
