摘要
目的研究心肌特异表达的新激酶基因p93在冠状动脉粥样硬化性心脏病(CAD)、扩张性心肌病(DCM)、先天性心脏病(CHD)和风湿性心脏病(RHD)中的作用。方法以杂交瘤技术制备p93单克隆抗体;应用酶联免疫吸附测定(ELISA)直接法检测正常人与上述4种疾病患者血清中p93的表达状况及血清浓度的差异。结果成功制备了人p93单克隆抗体,效价达1:100 000;正常人及CAD、DCM、CHD、RHD患者血清中均有p93的表达;正常人血清水平对数值为2．32±0．19;4种疾病患者的p93血清水平(对数值分别为2．46±0．22,2．47±0．20,2．58±0．26,2．49±0．19)均高于正常人(P<0．05)。其中CHD组明显高于正常对照组及CAD组(P<0．05)。结论p93基因与这4种疾病均有密切关系,以CHD尤为突出。推测该基因可能在上述各疾病的发生、发展过程中起着重要作用,但其机制亟待进一步探讨。
Objective To study the function of p93 gene, a novel cardiac-specific kinase, in coronary artery disease （ CAD ）, dilated cardiomyopathy （ DCM ）, congenital heart disease （ CHD ） and rheumatic heart disease （RHD）, Methods The monoclonal antibodies （McAb） against human p93 gene were obtained through hybridoma method, The serum levels of p93 in the normal human and the four diseases patients were detected by Enzyme-linked immunosorbent assay（ELISA） , they were also compared with each other, Results The p93-McAb was successfully obtained with titer 1：100 000, The p93 antigen can be detected in the serum of normal human and the patients. The serum levels of CAD, DCM, CHD, RHD patients were 2. 46 ± 0. 22,2. 47 ± 0. 20,2. 58 ± 0. 26 and 2. 49 ± 0. 19 and 2. 494 1± 0. 192 61 （ logarithm ）, and they were higher than that of normal human （P 〈0. 05） whose logarithm was 2. 32 ±0. 19. CHD group was significantly higher than normal human and CAD group （ P 〈 0. 05 ）. Conclusions The p93 gene is closdy associated four cardiovascular diseases, especially with the CHD. It can be speculated that the gene may play an important role in the appearance and development of these diseases, but its mechanism needs further investigation.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2006年第8期704-707,共4页
Chinese Journal of Laboratory Medicine
基金
国家自然科学基金资助项目(30470724 30300131)
国家自然科学青年基金资助项目(30500200)
关键词
心血管疾病
基因
酶联免疫吸附测定
细胞凋亡
Cardiovascular diseases
Gene
Enzyme-linked immunosorbent assay
Apoptosis
