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生物法净化苯、甲苯、二甲苯废气菌种驯化 被引量:6

Acclimation of High Biodegradability Consortium of Benzene Toluene and Xylene in a Biotrickling Filter
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摘要 对生物滴滤器净化苯、甲苯、二甲苯废气菌种驯化进行了研究,创建了“诱导物-目标污染物”专性降解菌双底物驯化模式,以甲苯为诱导物,进行了“甲苯-苯”、“甲苯-二甲苯”双底物驯化,并将苯专性降解菌接种于Ф40mm×500mm的生物滴滤器进行净化笨的工艺实验.结果表明:“甲苯-苯”驯化第25天时,其假单胞杆菌密度高达2.08×10^8CFU/mL,比苯直接驯化高出约5×10^7CFU/mL;“甲苯-二甲苯”驯化第25天时,其假单胞杆菌密度为1.60×10^8CFU/mL;净化苯试验表明,气量为60L/h,苯的质量浓度为0.747~3.760mg/L条件下,苯平均去除能力高于120g/(m^3·h),峰值去除能力达到246g/(m^3·h)(此时假单胞杆菌密度高达6.0×10^10CFU/mL),采用双底物驯化方式优于直接驯化. In order to develop the effective controlling technology for emission of Benzene, Toluene and Xylene (BTX), the microflora acclimation for the purification of BTX was studied. A novel acclimation method, called inducer-target graded acclimation, was proposed, in which the toluene was used as the inducer substrate, the benzene and xylene were target ones. The toluene-benzene and toluene-xylene dual substrates were employed to acclimate the benzene and xylene biodegradation consortium. The benzene-removal experiment was performed on the bench- scale biofilter trickling system of Ф40 mm× 500 mm for validating this acclimation method. The results of Benzene-removal experiments show that when the gas flux of 60 L/h and benzene concentration in inlet gas of 0. 747-3.760 mg/L, the maximum biological elimination capacity of benzene in waste gas of 246 g/(m^3 · h) and the steady biological elimination capacity of over 120 g/(m^3 · h) were achieved. The Pseudomonas putida is the dominant bacterial and the density amounts to 6.0 × 10^10 CFU/mL according to the observation for biotic community.
出处 《中国矿业大学学报》 EI CAS CSCD 北大核心 2006年第5期633-636,641,共5页 Journal of China University of Mining & Technology
基金 江苏省自然科学基金项目(BK2004031) 江苏省环境科技发展基金项目(2004007) 中国矿业大学科学研究基金项目(0P4069)
关键词 生物滴滤器 BTX苯 双底物驯化 去除能力 biotrickling filter BTX benzene dual substrates acclimation elimination capacity
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