摘要
针对不同物种采用不同方法培养小鼠、山羊和鸡输卵管上皮细胞(OECs)并检测上皮细胞纯度,在此基础上比较了它们支持小鼠胚胎发育的能力。结果表明,3种细胞都能很好地生长,培养的细胞几乎全为上皮细胞(Vi-mentin单克隆抗体检测显示,小鼠、山羊和鸡OECs的阳性细胞率分别为2.4±0.3、1.3±0.3和1.8±0.4)。昆明白小鼠原核胚与小鼠、山羊和鸡OECs共培养,发育到囊胚的比例分别为61.7%、57.2%和56.2%,差异不显著(P>0.05)。说明鸡和山羊OECs能很好地支持小鼠胚胎发育。
Oviductal epithelial cells （OECs） of mouse , goat and chick were cultured by different methods and examined percents of Vimentin positive cells by Vimentin monoclonal antibody, then on this basis in vitro development of mouse pronuclear embryos co-cultured with them were compared. The results are summarized as follows： Somatic cells grow well in culture system and almost all are epithelial cells. Percents of Vimentin positive cells of mouse , goat and chick OECs were 2.4±0.3, 1.3 ± 0.3 and 1.8 ± 0.4, respectively. The proportion of pronuclear embryos from Kunming mouse developing to blastocysts （61.7% ,57.2% and 56.2%, respectively） did not differ when co-cultured with primary mouse, goat and chick OECs, suggesting that a good development of mouse embryos was also achieved when co-cultured with goat and chick OECs.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2006年第9期878-882,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金资助项目(30170679
30430530
30571337)
国家"973"计划项目(G200016108)
关键词
胚胎
体细胞
共培养
小鼠
embryos
somatic cells
co-culture
mouse
