摘要
目的:比较回阳生肌膏提取物对人正常皮肤成纤维细胞(nHFB)及慢性溃疡创缘成纤维细胞(uHFB)增殖的影响。方法:组织块法体外培养nHFB及uHFB;采用Alarmarblue摄入法观察不同浓度回阳生肌膏水提取物、醇提取物对两种细胞增殖的影响;并采用流式细胞仪检测其水提取物、醇提取物对两种细胞细胞周期的影响。结果:回阳生肌膏水提取物、醇提取物分别作用上述两种细胞24h后,细胞增殖速度均增加。回阳生肌膏醇提物分别在0.027-0.425mg/L和0.027-0.213mg/L范围对nHFB和uHFB的增殖有促进作用(P<0.05或P<0.01);回阳生肌膏水提物分别在0.075-2.400mg/L和0.150-1.200mg/L范围对nHFB和uHFB增殖有明显促进(P<0.05或P<0.01);流式细胞仪检测结果显示,对于nHFB,水提物作用24h后S期比例明显上升(Pμ<0.01),G2/M期比例无明显升高,细胞增殖指数(PrI)明显增加(P<0.01),醇提物作用24h后S期、G2/M期比例均上升(P<0.05),细胞增殖指数(PrI)明显增加(P<0.01);而对于uHFB,水提物作用24h后S期、G2/M比例上升均不显著,但PrI指数有增加(P<0.05),醇提物作用24h后S期比例明显上升(P<0.01),G2/M期比例无明显升高,PrI明显增加(P<0.01)。结论:回阳生肌膏无论水提取物、醇提取物皆可不同程度促进nHFB及uHFB的增殖,其醇提物作用更迅速;这种促增殖作用可能是通过调节细胞周期使更多细胞进入S期而实现的。
AIM： To compare the effects of alcohol and aqueous extracts from Huiyang shengji gao （HYSJG） , a Chinese medicine, on proliferation of human fibroblasts derived from normal and ulcer margin skin. METHODS： Using explant culture techniques, we obtained populations of normal human fibroblast （nHFB） and ulcer marginal fibroblast （uHFB）. Alarmar blue assay was used for observing cell proliferation, and flow cytometry for cell cycle analysis. RESULTS： After cultured with aqueous extracts and alcohol extracts of HYSJG for 24 hours, the proliferation rates of the two cell lines were both elevated. For alcohol extract, obvious proliferation - promoting concentrations on nHFB and uHFB were 0. 027 -0. 425 mg/L and 0. 027 - 0. 213 rag/L, respectively （ P 〈 0. 05 or P 〈 0. 01 ）. For aqueous extract, they were 0. 075 - 2. 400 mg/L and 0. 150 - 1. 200 mg/L, respectively （ P 〈 0. 05 or P 〈 0. 01 ）. Aqueous extract increased the percentage of populations of S phase and cell proliferation index （PrI, S ＋ GJM） significantly （P 〈0. 01 vs nHFB control, respectively）. Besides S phase and PrI increased, alcohol extract also enhanced GJM phase of nHFB （P 〈 0. 05 vs nHFB control）. Exposure of uHFB to alcohol extract, the percentage of S phase increased （P 〈 0. 01 vs uHFB control） , but GJM phase had unobvious changes, the PrI increased （P 〈 0. 01 vs uHFB control）. Aquaeous extract added to uHFB, the PrI increased dramaticaly （P 〈0. 01 vs uHFB control）, but the percentage of S and GJM phase didn＇t show obvious increase. CONCLUSION： Aqueous extract and alcohol extract from HYSJG accelerate nFB and uFB proliferation by promoting more cells in G0/GI phase into S phase and onward further.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第9期1808-1810,共3页
Chinese Journal of Pathophysiology
基金
北京市中医药科技项目(No. JJ2003 -13)
关键词
成纤维细胞
中草药
溃疡
Fibroblasts
Drugs, Chinese
herbal
Ulcer
