摘要
用电子自旋共振（ＥＳＲ）技术和荧光偏振技术，对烧伤焦痂脂类提取物（Ｄ１）直接作用于大鼠肝线粒体前后的膜脂流动性作了测定。结果：Ｄ１引起马来酰亚胺（ＭＡＬ－Ⅰ）标记的线粒体膜蛋白的Ｓ／Ｗ值下降；Ｄ１使５－Ｄｏｘｙｌ标记的线粒体膜脂Ｓ值降低；并使肝线粒体的荧光强度（Ｆ）升高，荧光偏振度（Ｐ）与各向异性（ｒ）下降。
The effects of D 1, a toxic substance isolated from burn eschar, on mitochondrial membrane fluidity were studied using the techniques of ESR spin label and fluorescence polarography in the rats. It was found that D 1 caused decrease of S/W ratio in 5 Doxyl labeling experiment. It also caused the lowering of degree of polarization fluorescence P on anisotrophy r. Both of these findings suggest increase of mitodrondrial membrane fluidity.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1996年第5期436-438,共3页
Journal of Third Military Medical University
基金
国家自然科学基金
关键词
灼伤
焦痂
膜流动性
肝线粒体
Burn
eschar
membrane fluidity
ESR
polarization fluorecence
