摘要
目的:探讨益气化瘀方对椎间盘软骨细胞凋亡状态下Bax、Bcl-2及Caspase-8表达的调控作用。方法:清洁级雄性1月龄SD大鼠20只,酶消化法获取椎间盘软骨细胞,用抗Fas抗体诱导其发生凋亡,通过益气化瘀方及其拆方益气方、化瘀方药理血清干预,免疫组化法(SP)检测分析大鼠椎间盘软骨细胞不同状态下Bax、Bcl-2及Caspase-8的表达。结果:相对正常组,凋亡模型组的Bax和Caspase-8表达明显,而Bcl-2轻度表达,有显著差异(P<0·01)。益气化瘀方、益气方和化瘀方均能降低椎间盘软骨细胞的Bax、Caspase-8的表达,而上调Bcl-2的表达,3种中药组与诱导凋亡组相比有显著差异(P<0·01)。结论:中药益气化瘀方、益气方和化瘀方都可以调控凋亡的大鼠椎间盘软骨细胞内Bax、Bcl-2、Caspase-8的表达,其治疗颈椎病的机制与其对凋亡相关因子的调控有关。
Objective： To study the effects of Yiqi-Huayu recipe （益气化瘀方） and its decomposed formulas-medicated on expressions of Bcl-2, Bax and Caspase-8 of apoptotic discus intervertebrale chondrocytes in rats. Methods： Immunohistochemical and integral optical density analytic methods were used to observe the effects of Yiqi-Huayu recipe, Yiqi recipe, Huayu recipe-medicated sera on the expressions of Bcl-2, Bax and Caspase-8 of apoptotic discus intervertebrale chondrocytes in rats induced by anti-Fas antibody. Results： As compared with apoptosis group, Bcl-2 expression was higher, Bax and Caspase-8 expressions were lower in Yiqi-Huayu-treated, Yiqi-treated, Huayu-treated （ P 〈 0.01 ）. Conclusion： Yiqi-Huayu recipe and its decomposed formulas can delay degeneration of the cervical intervertebral disc, which may be due to its action in regulating the expressions of Bcl-2, Bax and Caspase-8.
出处
《中国骨伤》
CAS
2006年第8期449-451,共3页
China Journal of Orthopaedics and Traumatology
基金
国家自然科学基金项目(编号:30171170&30572398)
国家自然科学基金重点项目(编号:0330700)
上海市科技"启明星"跟踪计划(编号:05QMH1412)
上海市医学重点学科建设项目(编号:05Ⅲ027)
上海市重点学科建设项目(编号:T0303)
关键词
椎间盘
软骨细胞
细胞凋亡
益气化瘀方
Intervertebral disc
Chondrocytes
Apoptosis
Yiqi-Huayu recipe
