摘要
背景与目的探讨绞股蓝提取物对衰老大鼠DNA氧化及烷化损伤的影响。材料与方法成年大鼠颈背部皮下注射D-半乳糖100mg/kg构建衰老动物模型,同时设注射等量生理盐水的正常对照组。已建模的大鼠分为人工合成饲料中添加不同剂量的绞股蓝提取物(200、800、4000mg/kg饲料)及维生素E、C(VE+C)各组,8周后采血获取淋巴细胞,用单细胞凝胶电泳法检测淋巴细胞DNA自发损伤及H2O2诱导的氧化损伤;实验结束前1周留取大鼠24h尿液,通过毛细管电泳法检测大鼠尿中O6-甲基鸟嘌呤(O6-MeG)的含量。结果各组大鼠淋巴细胞DNA自发损伤无明显差异(P>0.05)。分别采用5、10和25μmol/LH2O2氧化时,绞股蓝各组及维生素E、C(VE+C)组DNA氧化损伤均明显低于衰老对照组(P<0.05),且绞股蓝800、4000mg/kg组DNA氧化损伤与正常对照组及VE+C组无显著性差别。衰老对照组尿中DNA烷化损伤代谢产物O-6MeG含量较其他各组偏高,但差别无统计学意义。结论D-半乳糖诱导衰老的大鼠模型DNA抗氧化损伤的能力降低,而DNA烷化损伤无明显改变;在本实验条件下,绞股蓝提取物可有效降低H2O2诱导的DNA氧化损伤,对DNA烷化损伤没有明显作用。
BACKGROUND ＆ AIM： To investigate the effects of Gynostemma pentaphyllum （GP） extract on DNA oxidative and alkylating damages. MATERIAL AND METHODS：： Adult Wistar rats received 100 mg/kg D-galactose subcutaneous injection each day to build the aging model. There was also a control group. These groups of rats were fed with fully compounded diet which was supplemented with different doses of Gynostemma pentaphyllum extract or vitamin E, C（VE ＋C） for 8 weeks. Whole blood was collected at the end of the trial. Spontaneous and induced oxidative DNA damages were analyzed by SCGE. Urine was collected before the end of the trial. Urinary O^6-MEG was analyzed by high performance capillary zone electrophoresis. RESULTS： There was no statistical difference in spontaneous DNA damage among 6 groups（P〉 0.05） . But less oxidative DNA damage induced by 5, 10, 25 μmol/L H2O2 were found in the groups supplemented with Gynostemma pentaphyllum extract and the VE ＋C group than in the aging group（P 〈0.05） .When supplemented with higher dose of Gynostemma pentaphyllum extract, DNA damage showed no significant differences compared with the control group and VE ＋C group. The level of urinary O^6-MEG in the aging group was not higher than that in the other groups （P 〉 0.05） . CONCLUSION： D-galactose could induce DNA oxidative effect on DNA alkylating damage.When Gynostemma pentaphyllum extract was given to aging damage induced by H2O2 could be effectively decreased and the alkylating DNA damage showed damage while it had no rats, the oxidative DNA no change.
出处
《癌变．畸变．突变》
CAS
CSCD
2006年第5期378-380,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家中医药局中医药科学技术研究专项基金(No:02-03ZP37)
青岛大学科研基金
关键词
绞股蓝
DNA损伤
彗星电泳
O^6-甲基鸟嘌呤
Gynostemma pentaphyllum
DNA damage
SCGE
O^6-MeG given to aging rats, the oxidative DNA damage showed no change
