摘要
目的观察不同浓度雌、雄激素对3T3-L1前脂肪细胞葡萄糖转运的影响,探讨性激素在胰岛素抵抗形成中的意义。方法体外培养3T3-L1前脂肪细胞,并诱导其分化成熟,利用2-脱氧-[3H]-D-葡萄糖掺入法,研究不同浓度的17β雌二醇、睾酮对胰岛素刺激的前脂肪细胞和脂肪细胞葡萄糖摄取能力的影响。结果10-8mol/L的17β雌二醇即能够抑制3T3-L1前脂肪细胞胰岛素刺激状态下的葡萄糖转运,且呈现明显的浓度依赖性抑制;而睾酮为10-8mol/L时3T3-L1前脂肪细胞胰岛素刺激状态下的葡萄糖转运并无明显影响,在浓度达到10-7mol/L开始出现抑制效应,浓度越高抑制效应越明显。结论性激素可以调节3T3-L1前脂肪细胞的胰岛素敏感性。
Objective To investigate the effect of different sex hormones on insulin-stimulated glucose uptake on cuhrured 3T3-L1 preadipocytes and adipocytes. Methods We treated cultured 3T3-L1 preadipocytes and adipo- cytes with different concentrations of 17β-Estradiol or testosterone and the effects were assessed as [ 3] 2-deoxy glu- cose uptake. Results After incubation with 17β-Estradiol or testosterone, the ability of insulin-stimulated glucose transport was statistically significantly reduced. Dosage response study demonstrated that 10^-7 mol/L testosterone or 10^-8 mol/L 17β-Estradiol can induce insulin resistance （P 〈0.01, respectively） in 3T3-L1 preadipocytes and adipocytes. The inhibition effect was in a dose-dependent manner. Condusion Sex hormone can regulate the insulin sensitivity of 3T3-L1 preadipocytes and adipocytes.
出处
《基础医学与临床》
CSCD
北大核心
2006年第9期980-983,共4页
Basic and Clinical Medicine
