摘要
目的:探讨淋巴管内皮细胞透明质酸受体-1(LYVE-1)在胃癌组织中的表达及临床意义．方法:采用组织芯片技术和免疫组化方法对85例胃癌组织和40例正常胃组织中LYVE-1的表达及淋巴管密度(LVD)进行检测．结果:LYVE-1的阳性产物表达于淋巴管内皮细胞胞质中,呈棕黄色染色．胃癌组织中LYVE-1阳性LVD显著高于正常胃组织(7．89±2．14 vs 1．15±1．62,P<0．01);瘤周的LYVE-1阳性LVD明显高于瘤内组织(9．28±1．18 vs 4．75±1．19,P<0．01);低分化(9．21±2．32)、Ⅲ-Ⅳ期(9．46±2．45)、有淋巴结转移(9．37±3．39)和远处转移(9．55±3．50)的胃癌组织中LYVE-1阳性LVD分别比高中分化(7．56±2．24)、Ⅰ-Ⅱ期(7．58±2．36)、无淋巴结转移(7．23±2．74)和远处转移(7．35±2．25)的明显增高(P<0．01, P<0．05)．结论:胃癌组织内及瘤周的LYVE-1阳性LVD明显增高,提示胃癌组织内的新生淋巴管主要位于瘤周,从而促进了肿瘤淋巴道转移．应用组织芯片大规模高效检测临床组织标本是可行的,具有快速、方便、经济、准确的特点．
AIM： To investigate the expression of lymphatic vessel endothelial hyaluronan receptor-1 （LYVE-1） and its clinical significance in gastric cancer. METHODS： Tissue microarray technique and immunohistochemistry were used to detect the expression of LYVE-1 and lymphatic vessel density （LVD） in 85 cases of gastric cancer tissues and 40 cases of normal gastric tissues. RESULTS： LYVE-1 was expressed in the endothelial cytoplasm of lymphatic vessels, showing as brown and yellow staining. The values of LYVE-1-positive LVD in gastric cancer and peritumoral tissues were significantly higher than those in normal gastric and intratumoral tissues （7.89 ± 2.14 vs 1.15 ± 1.62, P 〈 0.01; 9.28 ± 1.18 vs 4.75 ± 1.19, P 〈 0.01）. In gastric cancer tissue, the value of LYVE-1-positive LVD was markedly higher in the cases with low differentiation （9.21 ± 2.32）, clinical stage Ⅲ-Ⅳ（9.46 ± 2.45）, lymph node metastasis （9.37 ± 3.39） or distant metastasis （9.55 ± 3.50） than that in the ones with well and moderate differentiation （7.56 ± 2.24）, clinical stage Ⅰ-Ⅱ（7.58 ± 2.36）, and without lymph node metastasis （7.23 ± 2.74） or distant metastasis （7.35 ± 2.25）, respectively （P 〈 0.01 or P 〈 0.05）. CONCLUSION： The high value of LYVE-1- positive LVD in gastric cancer and peritumoral tussues indicates that lymphatic angiogenesis mainly locates in peritumoral tussues, which promotes lymphatic metastasis. Tissue microarray is a feasible, rapid, economic and accurate approach for screening clinical tissue specimens in a large scale.
出处
《世界华人消化杂志》
CAS
北大核心
2006年第24期2421-2424,共4页
World Chinese Journal of Digestology
关键词
胃肿瘤
LYVE-1
淋巴管生成
淋巴管密度
组织芯片
Gastric neoplasms
Lymphatic vesselendothelial hyaluronan receptor-i
Lymphatic angiogenesis
Lymphatic vessel density
Tissue microarray
