摘要
用一步法和准一步法合成了以三羟甲基丙烷为核的两个系列的超支化聚酯，利用红外光谱、羟值测定等手段对其分子结构进行了表征。利用超支化聚合物低粘度的特点，采用化学键合的方法将其涂于石英毛细管电泳柱内壁，使其在毛细管内壁上形成稳定的超支化聚酯涂层。该涂层在pH3．0—7．0范围内能够有效地抑制电渗流和碱性蛋白质在毛细管壁上的吸附。实验结果表明：该涂层柱在pH5．0的磷酸缓冲溶液中，对碱性蛋白质的分离柱效可高达塔板数10^6／m。每次运行之间（n=6），天与天之间（n=3），以及柱与柱之间（N=3）的迁移时间的标准偏差（RSD％）在0．5％-1．5％之间，表明本方法制得的涂层柱具有良好的稳定性。
Two series of hyperbranched polyesters with trimethylolpropane as core were synthesized using one-step and pseudo-step procedure, and the molecular structures of them were characterized by infrared spectra （IR） and hydroxyl value. They were coated and formed steady coatings on the inner surface of the fusedsilica capillaries in chemically-bond Way. The coatings could reduce the electroosmotic flow greatly and suppress protein adsorption between the inner surface of the fused-silica capillaries and the basic proteins effectively in the pH range of 3 -7. The separation efficiency of 106 plates/m was obtained. Good reproducibility （ RSD 〈 1.5 % ） of migration time from run to run, day to day and column to column was also obtained.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2006年第9期1287-1290,共4页
Chinese Journal of Analytical Chemistry
关键词
毛细管电泳
蛋白质
涂层柱
超支化聚酯
Capillary electrophoresis, protein, coated column, hyperbranched polyesters
