摘要
研究了在铂丝电极上电聚合一层带正电的2-氨基吡啶膜。然后再利用层层自组装技术固定纳米金、电子媒介体硫堇及辣根过氧化氢酶，从而制备了由辣根过氧化氢酶／纳米金／硫堇／纳米金／聚2-氨基吡啶膜修饰的酶生物传感器．实验巾探讨了聚合层数、温度、pH值等对电极响应的影响．结果表明该传感器在H2O2浓度6．0×10^-7～1．3×10^-3mol／L范围内呈线性响应，检出限为2．1×10^-7mol／L．此外，该传感器具有好的稳定性和选择性，能有效排除抗坏血酸、柠檬酸、葡萄糖等常见物质的干扰．
A novel hydrogen peroxide blosensor has ted by the self-assembly technique on the electrode been prepared. HRP/nano-Au/thlonine/nano-Au were construcmodified by poly 2-amlnopyrldine, which was electropolymerized onto a platinum（Pt） electrode to form a positively charged surface. The negatively charged nano-Au absorbed on poly 2-aminopyridine was employed as matrix to immobilize thionlne as an electron mediator and horseradish peroxidase. The H2O2 biosensor prepared displayed a quick and sensitive response to hydrogen peroxide. The linear response of detection of the biosensor to hydrogen peroxide is in the concentration range of 6.0 × 10^-7 - 1.3 × 10^-3 mol/L with a detection limit of 2. 1 × 10^-7 mol/L. Moreover, the studied biosensor exhibited good repeatability and high selectivity and it can effectively remove the interference of such common matter as the ascorbic acid citric acid glucose, etc.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第5期66-72,共7页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(29705001)
重庆市自然科学基金资助项目(CSTC 2004BB4149 ,2005BB4100)
西南师范大学博士基金资助项目(SWNUB2004021) .
