摘要
工程菌308R(pKSH)携带有梨火疫欧文氏杆菌的hrpN基因,能产生并分泌诱导植物抗病性蛋白-Harpin。该工程菌在无选择压培养基中生长50代,带有重组质粒pKSH的细胞占总菌量的23·1%,对照菌308R(pCPP430)的细胞占4·75%。将工程菌和对照菌喷雾到番茄叶面,保湿条件下的13d内,叶面菌量维持在105cfu/cm2以上,自然条件下的5d内,菌量维持在104cfu/cm2以上,其间308R(pKSH)的稳定性一直高于对照菌。因此证明工程菌308R(pKSH)比对照菌308R(pCPP430)稳定性有所提高,但还是不够理想。讨论了该工程菌不稳定的原因以及改进途径。
Biocontrol bacteria 308R （pKSH） with hrpN ofErwinia amyloyora. It can secrete the Harpin protein which can induce plant resistance. After successive growth in antibiotic-free LB medium for 50 generations, only 23.1% of the cell still retained the plasmid pKSH, in comparison with 4. 75% of the cosmid pCPP430. Sprayed onto the leaves of tomato, the recombinant strain maintained a population density of over 10^5 cfu/cm^2 when kept under high humidity in thirteen days, hut if without humidity, the strain keep 10^4cfu/cm^2 over in five days. On the tomato leaves, the stability of the recombinant strain 308R （ pKSH ） was higher than the strain 308R （pCPP430） . The experiment indicated that the recombinant strain 308R （pKSH） had higher stability compared with the strain 308R （ pCPP430）, but that was not enough. In this paper reasons for unstability and strategies for improving were discussed for the recombinant strain.
出处
《微生物学通报》
CAS
CSCD
北大核心
2006年第5期50-53,共4页
Microbiology China
基金
山西省自然科学基金资助项目(No.20021080)
山西省科技攻关计划项目(No.2006031047)
