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结核病血清学诊断进展 被引量：5

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摘要 结核病的细菌学检查目前是结核病实验室诊断的金标准。由于痰涂片阳性率较低，镜检需要经验，难以区分环境分枝杆菌造成的假阳性；痰培养需时太长，因此细菌学检查对结核病的诊断价值有限。随着分子生物学技术的迅速发展，用高度敏感的方法检测结核分枝杆菌（简称结核杆菌）及其特异性DNA片段，如聚合酶链反应（PCR）、生物探针和基因芯片等，需要相应的检测设备和检测费用高而未能广泛推广。
作者 刘来成 卢贤瑜 范雄林
机构地区 重庆医科大学临床免疫学教研室 华中科技大学同济医学院微生物学教研室
出处 《微生物与感染》 2006年第3期170-173,共4页 Journal of Microbes and Infections
关键词 结核病 血清学诊断 结核分枝杆菌 分子生物学技术 细菌学检查 聚合酶链反应 检测设备 实验室诊断
分类号 R52 [医药卫生—内科学]
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参考文献19

  • 1Antunes A, Nina J, David S. Serological screening for tuberculosis in the community: an evaluation of the Mycodot procedure in an African population with high HIV-2 prevalence (Republic of Guinea-Bissau). Res Microbiol, 2002, 153 : 301- 305
  • 2Wu HP, Shieh WB, Hsien FK, et al. The significance of mycobacterium tuberculosis antibody, antigen 60 IgG in patients with abnormal chest radiography. Chang Gung Med J,2004,27:869 - 876
  • 3Maekura R, Kohno H, Hirotani A, et al . Prospective clinical evaluation of the serologic tuberculous glycolipid test in combination with the nucleic acid amplification test. J Clin Microbiol, 2003,41 : 1322 - 1325
  • 4Tiwari RP, Tiwari D, Garg SK, et al. Glycolipids of Mycobacterium tuberculosis strain H37Rv are potential serological markers for diagnosis of active tuberculosis. Clin Diagn Lab Immunol,2005,12:465 - 473
  • 5Butt T, Malik HS, Abbassi SA, et al. Genus and species-specific IgG and IgM antibodies for pulmonary tuberculosis. J Coll Physicians Surg Pak,2004, 14:105 - 107
  • 6Houghton RL, Lodes MJ, Dillon DC, et al. Use of multiepitope polyproteins in serodiagnosis of active tuberculosis. Clin Diagn Lab Immunol, 2002,9: 883 - 891
  • 7Wilson RA, Maughan WN, Kremer L, et al. The structure of Mycobacterium tuberculosis MFF51 (FbpC1) defines a new family of non-catalytic alpha/beta hydrolases. J Mol Biol,2004,335:519 - 530
  • 8Kashyap RS, Dobos KM, Belisle JT, et al. Demonstration of components of antigen 85 complex in cerebrospinal fluid of tuberculous meningitis patient. Clin Diagn Lab Immunol, 2005,12: 752 - 758
  • 9Arend SM, de Haas P, Leyten E, et al. ESAT-6 and CFP-10 in clinical versus environmental isolates of Mycobacterium kansasii. J Infect Dis,2005,191 : 1301 - 1310
  • 10Pym AS, Brodin P, Brosch R, et al. Loss of RD1 contributed to the attenuation of the live tuberculosis vaccines Mycobacterium boris BCG and Mycobacterium microti. Mol Microbiol, 2002,46: 709 - 717

二级参考文献13

  • 1Elhay MJ,Oettinger T,Andersen P.Delayed-type hypersensitivity responses to ESAT-6 and MPT64 from Mycobacterium tuberculosis in the guinea pig.Infect Immun,1998,66:3454-3456.
  • 2Colangeli R,Spencer JS,Bifani P,et al.MTSA-10,the product of the Rv3874 gene of Mycobacterium tuberculosis,elicits tuberculosis-specific,delayed-type hypersensitivity in guinea pigs.Infect Immun,2000,68:990-993.
  • 3Brusasca PN,Colangeli R,Lyashchenko KP,et al.Immunological characterization of antigens encoded by the RD1 region of the Mycobacterium tuberculosis genome.Scand J Immunol,2001,54:448-452.
  • 4Dillon DC,Alderson MR,Day CH,et al.Molecular and immunological characterization of Mycobacterium tuberculosis CFP-10,an immunodiagnostic antigen missing in Mycobacterium bovis BCG.J Clin Microbiol,2000,38:3285-3290.
  • 5Sorensen AL,Nagai S,Houen G,et al.Purification and characterization of a low-molecular-mass T-cell antigen secreted by Mycobacterium tuberculosis.Infect Immun,1995,63:1710-1717.
  • 6Berthet FX,Rasmussen PB,Rosenkrands I,et al.A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein(CFP-10).Microbiology,1998,144(Pt 11):3195-3203.
  • 7Harboe M,Oettinger T,Wiker HG,et al.Evidence of occurrence of the ESAT-6 protein in Mycobacterium tuberculosis and virulent Mycobacterium bovis and for its absence in Mycobacterium bovis BCG.Infect Immun,1996,64:16-22.
  • 8Vordermeier HM,Whelan A,Cockle PJ,et al.Use of synthetic peptides derived from the antigens ESAT-6 and CFP-10 for differential diagnosis of bovine tuberculosis in cattle .Clin Diagn Lab Immunol,2001,8:571-578.
  • 9van Pinxteren LA,Ravn P,Agger EM,et al.Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP-10.Clin Diagn Lab Immunol,2000,7:155-160.
  • 10Munk ME,Arend SM,Brock I,et al.Use of ESAT-6 and CFP-10 antigens for diagnosis of extrapulmonary tuberculosis.J Infect Dis,2001,183:175-176.

共引文献16

同被引文献53

引证文献5

二级引证文献12

微生物与感染
2006年 第3期

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