摘要
目的:微波萃取法提取桑叶和桑白皮中黄酮类成分。方法:采用正交试验设计优选出微波萃取的最佳工艺条件,并与传统醇提法相比较,同时比较桑的不同部位药材的黄酮含量。结果:微波萃取黄酮类成分的最佳工艺条件为:70%乙醇12倍量,于60℃萃取20 m in,其中提取溶剂对结果有显著影响。桑叶中的黄酮含量约为桑白皮中的9倍,且应用微波萃取法比传统醇提法分别高55%和32%。结论:微波萃取法在提取时间、溶剂用量及得率等方面明显优于传统的醇提法,适于工业生产。
Objective： To extract flavonoids in Mulberry Leaf and White Mulberry Root-bark by microuave-assisted method. Methods： An orthogonal table was used to find the best procedure for microwave-assisted extraction （MAE） of flavonoids. The advantages of MAE were explored by comparing with the commonly used extraction methods. The different contents of flavonoids in Mulberry Leaf and Mulberry Root-bark were also compared. Results： The best condition for procedure was extracting 20 min at 60℃ with 12 times of 70% ethanol. Among all factors, the ethanol concentration was significant This experiment implied that flavonoids in Mulberry Leaf were 9 times as that in Mulberry Root-bark probably. Moreover, the flavonoids extracted from Mulberry Leaf and White Mulberry Root-bark by MAE were 55% and 32% more than that from commonly used extraction method individually. Conclusion： MAE is advantageous than commonly used extraction method, It can be used for batch production.
出处
《中药材》
CAS
CSCD
北大核心
2006年第10期1090-1092,共3页
Journal of Chinese Medicinal Materials
基金
浙江省分析测试基金资助项目(03047)
浙江大学城市学院科研基金资助项目(J56203041015)
关键词
微波萃取法
黄酮
正交试验
桑叶
桑白皮
Microwave-assisted extraction （MAE）
Flavonoids
Orthogonal table
Mulberry Leaf
White Mulberry Root-bark
