摘要
目的:从枸杞中提取分离枸杞多糖(LBP),并对其组成进行研究。方法:采用水提法提取LBP,用DE-AE纤维素柱色谱和凝胶柱色谱进行分离纯化,采用GPC-LLS法、气相色谱法、氨基酸自动分析等方法对其组成进行研究。结果:LBP纯品含有4个级分,LBP的分子质量约为1.524×105,由阿拉伯糖(Ara),鼠李糖(Rha),木糖(Xyl),甘露糖(Man),半乳糖(Gal)和葡萄糖(Glc)6种中性单糖和半乳糖醛酸及18种氨基酸组成,结论:LBP是酸性杂多糖同多肽或蛋白质构成的的复合性多糖,且存在Glycan-O-Ser的糖肽结构。
Objectove： To study the extraction, isolation and composition of Lycium barbarum polysaccharides （LBP）. Method： LBP was extracted from L. barbarum with water, isolationed and purified by DEAE ion-exchange cellulose and gel chromatography, and their structural composition was studied by means of SDS-PAGE gel electrophoresis, GC, amino acid automatic analysis, etc. Result： Pure LBP has four water soluble polysaccharides, M W was 1. 524×10^5. LBP was composed of 6 kinds of monosaccharides （Ara, Rha, Xyl, Man, Gal and Glc）, galacturonic acid and 18 kinds of amino acids. Conclusion： LBP is a kind of complex polysaccharides consisting of acidic heteropolysaccharides and polypeptide or protein, and LBP has Glycan-O-Ser glycopeptide structures.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2006年第19期1603-1607,共5页
China Journal of Chinese Materia Medica
基金
黑龙江省教育厅科研资助项目(10533008)
关键词
枸杞多糖
提取
分离
组成
Lycium barbarum polysaccharide
extraction
isolation
composition
