摘要
用腹腔注射的方法对实验小鼠进行SO2衍生物(亚硫酸钠和亚硫酸氢钠,分子比为3:1)染毒,染毒剂量分别为0,0.025,0.100,0.400g/(kg·d),共染毒5d.研究了SO2衍生物对小鼠脾、胃蛋白质的氧化损伤作用并探讨其分子作用机制.结果表明,经SO2衍生物处理,可以使小鼠脾、胃蛋白质的羰基含量增大,且染毒剂量与蛋白质的羰基含量之间呈现直线相关关系,相关系数分别为0.899和0.855(P=0.05).蛋白质羰基含量增大表现为脾>胃.说明SO2衍生物可使小鼠脾、胃组织蛋白质产生氧化损伤,氧化损伤的程度大小因组织而异.
The contaminative of SO2 derivatives （sodium sulfite and sodium bisulfite molecular ratio of 3：1） was carried out on test mice, using abdominal cavity inject techniques; the contamination doses were 0, 0.025, 0.100, 0.400g/（kg·d） for 5 days. The oxidative damage action of SO2 derivatives on the mice spleen and stomach proteins was studied, and its molecular action mechanism was probed into. SO2 derivative treating could increase the carbonyl content of protein of mice spleen and stomach; and appeared linear correlation between the contamination dose and the carbonyl content protein with correlation coefficients of 0,899 and 0.855 （P=0.05）, respectively. The increase of carbonyl content of protein expressed the order of spleen 〉stomach. This indicated that the SO2 derivatives could cause the oxidative damage of proteins of spleen and stomach tissues of mice, and the size of the oxidative damage degree differed with different tissues.
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2006年第5期595-598,共4页
China Environmental Science
基金
国家自然科学基金资助项目(30230310
20477023)
山西省自然科学基金资助项目(20031092
20021014)
关键词
二氧化硫
亚硫酸钠
蛋白质氧化损伤
羰基含量
脾
胃
sulfur dioxide
sodium sulfite
protein oxidative damage
carbonyl content, spleen
stomach
