摘要
目的建立高效液相色谱法（HPLC）测定人血浆中替米沙坦的血药浓度。方法采用高效液相色谱-荧光检测法，色谱为shima—Pak（C18，150mm×4．65mm，5μm），流动相为乙睛-磷酸盐缓冲液（45：55，pH5．8），流速为1.0mL／min,激发波长和发射波长分别为305nm和365nm，进样量10μL，内标物为α-萘酚，采用乙睛沉淀除蛋白的方法。结果替米沙坦在0.625～110μg／L范围内线性良好（r=0．9996），此方法精密度试验结果较好。结论HPLC法便捷，准确，灵敏，适于替米沙坦血药浓度测定和药动学研究。
Objective To establish an HPLC-fluorimetric method in determination of telmisartan in human plasma. Methods The chromatographic column was Hypersil C18column（150 mm ×4.6 mm 5 μm）. The mobile phase was acetonitrile-PH 5.8 phosphate buffer（45 ： 55） and the flow rate was 1.0 mL/min, The fluorimetric excitation and emission wave lengths were set at 305 nm and 365 nm, respectively, The internal standard α-naphthol, Blood samples were deproteined by adding acetonitrile. The injection volume was 10μL. Results The linearity correlation of telmisartan ranked from 2 to 110μg/L（r=0. 9996） and the Precision and stability of the method were fine. Conclusion This is a convenient,sensitive,accurate and reproducible method for determining the concentrations of telmisartan in plasma and investigating its pharmacokinetics.
出处
《江西医学院学报》
CAS
2006年第5期33-34,36,共3页
Acta Academiae Medicinae Jiangxi
