摘要
对５１例人体尸检材料及４０只ＳＤ大白鼠不同损伤时间组标本进行组织内表皮生长因子受体（ＥＧＦＲ）的酶标染色，结果发现：生前不同方式损伤类型，ＥＧＦＲ阳性染色率在不同损伤时间表达不同。刀伤、枪伤ＥＧＦＲ阳性染色率为７０．０％－８０．０％，而挫伤和索沟皮损的ＥＧＦＲ阳性染色率较低，仅为４．０％－１０．０％左右。动物实验结果提示，损伤５分钟至３０分钟；ＥＤＦＲ阳性率较低，仅为４００％，从０．５小时至２小时、阳性染色率明显提高，为６０．０％～１００．０％，我们实验还发现，ＥＧＦＲ在人体和动物死后伤均为阴性染色。故此，可用其作为法医生前和死后伤参考指标。
The immunohistochemical mathod (SP) has been used to examine the distribution of EGFR in different mechanical injures of skin (51 cases human. 40 casec rat). The results showed that:(1) The positive rates of EGFR in the different types of injures are different. Incised and gunshot wound are 70.0% to 80.0%. contused wound and ligature furrow are lower than above. only about 4.0%-10.0%. (2)Staining patterns of EGFR were observed from diffenrent time groups of rats (5 min, 10 min, 15 min, 30 min, 1hr., 1.5hr, 2 hr, after injury). The positive rates expressed low at 5 min to 30min (40%) and high at 0.5 hr. to 2hr. (60.0% to 100.0%).(3) Normal skin and postmortem wound both human and rats can't be stained by EGFR.
出处
《法医学杂志》
CAS
CSCD
1996年第3期136-138,共3页
Journal of Forensic Medicine
