摘要
目的了解湖北地区幽门螺杆菌(Hp)空泡毒素基因(vacA)不同亚型及其与慢性胃炎(CG)和慢性消化性溃疡(CPU)之间的相关性。方法采用共享引物PCR技术和特异的PCR分型引物,对74例CG或CPU患者胃黏膜活检组织Hp分离株进行分型,并分析不同亚型与这两种疾病的相关性。结果湖北地区的Hp vacA基因主要以s1a-m 2型为主,vacA的优势亚型分布与上海和广州相似,除了s2型以外,s1a-m 1、s1b-m 1、s1b-m 2、s1c-m 1和s1c-m 2均有分布;另有8株不能被完全分型。结论湖北地区Hp vacA基因的不同亚型与CG和CPU之间不存在相关性,CG和CPU的引发因素有待进一步研究。
[Objective]To study the vacuolating cytotoxin gene A （vacA） subtype of Helicobacter pylori （Hp） distributed in Hubei area, and to explore the association between different vacA subtypes and chronic gastritis （CG） and chronic peptic ulcer （CPU）. [Methods]The shared-primer PCR technique and specific PCR primers were used in our research to subtype the vacA gene of Hp strains isolated from gastric mucosal biopsies of 74 cases and the association between the vacA sub-genotypes and their clinical outcomes was evaluated by SAS statistical soft ware, [Results]It was primarily sla-m2 in Hubei area and the dominant vacA subtypes of prevalent strains in Hubei area that were similar to those in Shanghai and Guangzhou area. In addition to s1a-m2, there were other subtypes like s1a-m1,s1b-m1,s1b-m2, s1c-m1 and s1c-m2. Furthermore, 8 strains of all could not be subtyped. [Conclusion]There is no correlation between different genotype of vacA and distinct clinical outcomes in Hubei area,the further investigation and research are still needed.
出处
《山东医药》
CAS
北大核心
2006年第31期11-12,共2页
Shandong Medical Journal
关键词
螺杆菌
幽门
基因
空泡毒素
分型
Helicobacter pylori
vacuolating cytotoxin gene A
typing
