摘要
用质谱法研究了人参皂苷Rb1和Rd与细胞色素c的非共价相互作用,证明主-客体之间形成了多种化学计量比的复合物,用直接计算的方法得出人参皂苷Rb1和Rd与细胞色素c形成的非共价复合物的各级解离常数KD,用竞争体系验证了计算结果,二者结果相互吻合.
Electrospray ionization mass spectrometry was applied to investigating the noncovalent interaction between ginsenosides and cytochrome c. Several noncovalent complexes with different stoichiometric relationships were observed from the mass spectra. The competition experiment was performed to validate the results. The dissociation constants of the complexes obtained by different systems are consistent with each other.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第11期2061-2065,共5页
Chemical Journal of Chinese Universities
基金
吉林省科技发展计划项目(批准号:20050560)资助
关键词
电喷雾离子化质谱
人参皂苷
细胞色素C
非共价复合物
Electrospray ionization mass spectrometry
Ginsenoside
Cytochrome c
Noncovalent complex
