摘要
Hybrid family of Argopecten irradians irradians was created by fertilization between two northern bay scallop individuals.Two families were analyzed in this study.The first family,Pa-Pb,is a pair mating between two scallops named Pa and Pb,while the second one crossed by individuals of Y1 and P0.Marker inheritance and segregation were studied in the 10 progenies of each family by randomly amplified polymorphic DNA（RAPD） analysis.102 RAPD primers were first screened by parental animals of both families.Only the primers with polymorphisms between the two parental animals in each family were selected for further analysis.In both families,parents and 10 progeny were analyzed with selected primers.In family Pa-Pb,a total of 122 bands generated from 12 selected primers.37 of them were polymorphic between two parents.The maternal Pa of this family had 17 molecular markers while paternal Pb had 20 markers.In Y1-P0,95 bands were produced by 10 selected primers.32 bands were polymorphic between maternal Y1 and paternal P0,who had 17 and 15 molecular markers respectively.In both families,each progeny analyzed in this study had at least 8 maternal markers and 5 paternal markers.Based on segregation patterns at all markers analyzed,we concluded that none of the progeny analyzed were from self-fertilization,and one-way hybridization between two individuals was successful in both of the two bay scallop families.
Hybrid family of Argopecten irradians irradians was created by fertilization between two nortbem bay scallop individuals. Two families were analyzed in this study. The first family, Pa - Pb, is a pair mating between two scallops named Pa and Pb, while the second one crossed by individuals of Y1 and P0. Marker inheritance and segregation were studied in the 10 progenies of each family by randomly amplified polymorphic DNA （RAPD） analysis. 102 RAPD primers were fast screened by parental animals of both families. Only the primers with polymorphisms between the two parental animals in each family were selected for further analysis. In both families, parents and 10 progeny were analyzed with selected primers. In family Pa - Pb, a total of 122 bands generated from 12 selected primers. 37 of them were polymorphic between two parents. The maternal Pa of this family had 17 molecular markers while paternal Pb bad 20 markers. In Y1 - P0,95 bands were produced by 10 selected primers. 32 bands were polymorphic between maternal Y1 and paternal P0, who had 17 and 15 molecular markers respectively. In both families, each progeny analyzed in this study had at least 8 maternal markers and 5 paternal markers. Based on segregation patterns at all markers analyzed, we concluded that none of the progeny analyzed were from self-fertilization, and one-way hybridization between two individuals was successful in both of the two bay scallop families.
出处
《水产学报》
CAS
CSCD
北大核心
2006年第5期713-719,共7页
Journal of Fisheries of China
基金
山东省科技发展计划项目(022110107)
青岛市科技计划项目(03-1-HH-10)
中国科学院知识创新工程重要方向课题(ZKCX2-211)
国家杰出青年基金(39825121)
关键词
海湾扇贝
人工授精
杂交
家系
RAID
分子标记
Argopecten irradians irradians
fertilization
hybridization
family
RAPD
molecular marker
