摘要
利用CTAB-异硫氰酸胍法、改良CTAB法和RNAplant试剂盒法，从富含多糖和酚类物质的苹果组培苗继代苗中提取总RNA。结果表明CTAB．异硫氰酸胍法对苹果组培苗总RNA的提取有较好的效果，所得RNA的A260／A瑚大于2．0，A260／‰为1．8—2．0，产率在16．95μg／g左右。改良CTAB法和RNAplant试剂盒法提取的RNA均有少量DNA污染，但RNAplant试剂盒法比CTAB法更快捷，简便。
The CTAB-guanidinium method, modified CTAB method, and RNAplant Kit method were used to extract the total RNA from the plantlets of apple cultured in vitro, in which contain a lot of carbohydrates and phenolic compound. The CTAB-guanidinium method showed the best results in term of the quality and quantity of yielded RNA with the A260/A230 value of more than 2.0, A260/A2s0 ranged from 1.8 to 2.0, and the total RNA yield of 16.95μg/g. The modified CTAB and RNAplant Kit methods were a bit short in total RNA extraction because of a little DNA contamination. And the RNAplant Kit method showed advantages of fastness and convenience.
出处
《华北农学报》
CSCD
北大核心
2006年第B10期50-53,共4页
Acta Agriculturae Boreali-Sinica
基金
河北省自然科学基金(C2004000368)
河北农业大学博士
引进人才启动基金资助项目
