摘要
目的从调节脾脏树突状细胞(DC)活性的角度探讨卡巴胆碱在防治脓毒症中的作用机制。方法将雄性C57BL/6小鼠30只按随机数字表法均分为正常对照组、脓毒症组和卡巴胆碱组。腹腔注射脂多糖(LPS)制备小鼠脓毒症模型,用流式细胞仪检测DC表面分子CD11c、CD86和主要组织相容性抗原复合物(MHC)的表达;采用免疫组化标记技术检测白细胞介素1β(IL1β)与IL12p70的阳性细胞率。结果注射LPS后,脾脏DC数量增加,但与正常对照组比较差异无显著性;DC表面分子MHC和CD86表达以及IL1β和IL12p70阳性细胞率均较正常对照组增高,差异均有显著性(P均<0.05);与脓毒症组比较,卡巴胆碱组脾脏DC的数量没有明显变化,但表面分子MHC和CD86表达及IL1β和IL12p70阳性细胞率均明显降低,差异具有显著性(P均<0.05)。结论卡巴胆碱能降低DC活性,有助于减轻脓毒症早期过度的免疫反应和炎症反应。
Objective To explore the mechanism of the effect of carbachol in the treatment of sepsis at the angle of modulation of dendritic cell （DC） activity. Methods Thirty male C57BL/6 mice were randomized into three groups： normal control, sepsis and carbachol. The sepsis model of mice was reproduced by the injection of lipopolysaccharides （LPS）. The expression of interleukin - 1β（IL - 1β） and IL - 12 p70 positive ceils were measured by immunohistochemistry method. The changes in DC activity in the spleen of mice were studied using flow cytometry. Results In LPS group, the number of splenic DC was increased, but it was not statistically significant. The expression of DC molecules including major histocompability complex -Ⅱ（MHC-Ⅱ）, CD86 and the rate of positive ceils containing IL - 1β and IL - 12 p70 were significantly higher than those in normal controls （all P〈0.05）. While in carbachol treatment group, the number of splenic DC had no significant change, the expression of IL - 1β was down-regulated, dramatically. The expression of MHC-Ⅱ, CD86 and IL - 12 p70 were also lowered （all P〈0.05）. Conclusion Carbachol can lower the DC activity, suggesting that modulation of DC activity may lower extensive immune and inflammatory response in the early phases of sepsis.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2006年第11期684-686,F0003,共4页
Chinese Critical Care Medicine
基金
全军"十五"医学科研基金资助项目(01MA210)
