摘要
目的研究中药黄芩有效成分黄芩苷对脂多糖(LPS)抑制人牙周膜成纤维细胞(HPLFs)活性及对LPS损伤HPLFs超微结构的影响,并初步探讨其可能的作用机理。方法采用原代培养人牙周膜成纤维细胞技术、四唑盐(MTT)比色试验和透射电子显微镜技术(TEM)。结果LPS浓度为100μg/ml时明显抑制细胞活性,同时加入LPS和黄芩苷后,细胞活性明显增强(P<0.05)。100μg/mlLPS对HPLFs各超微结构均有不同程度的损伤,特别是线粒体损伤严重,同时加入LPS和黄芩苷(10μg/ml)后,与LPS组比较,超微结构损伤减轻,细胞器结构表现也较正常。结论中药黄芩苷能显著促进成纤维细胞的增殖,对LPS抑制HPLFs活性和LPS损伤HPLFs超微结构具有保护作用,其作用机制可能与降解LPS及作用于细胞膜表面阻止LPS进入细胞内部有关。
Objective To observe the effects of the Chinese baicalin on LPS which lead to the decreases of activities and ultrastructural, impairments of human periodontal ligament fibroblasts （HPLFs）, and try to study the mechanism of the effects. Methods The cell culture technique in vitro, thiazolyl blue tetrazolium bromide （MTT） staining and spectrophotometric assay and transmission electron microscope （TEM） method were used to observe the effects of baicalin on LPS. Results At the concentration of 100μg/mL, LPS of E. Coli could inhibit the proliferation of activities of HPLFs remarkably and proliferation of activities of HPLFs improved greatly after adding baicalin. Furthermore, LPS had significant impairmental effect on ultrastrutures of HPLFs in 100μg/ml, especially on the rnitochondria. After adding baicalin, the impairment of ultrastructures of HPLFs was decreased. Conclusion The Chinese baicalin has the protective effects on the fibroblasts of human periodontal ligament induced by LPS. The mechanism of the effects may be related to the decomposition of LPS and the protection of the Chinese baicalin which prevents LPS from going into HPLFs.
出处
《现代口腔医学杂志》
CAS
CSCD
北大核心
2006年第6期634-637,共4页
Journal of Modern Stomatology
