摘要
目的—对一例不同寻常的白变猕猴进行病因研究,以探讨其色素病变的分子机理。方法—利用DH-PLC分析及测序技术对酪氨酸酶基因进行突变研究。并检测了毛发黑色素含量、微量元素含量等;结果—白猴血液指标无异常变化,酪氨酸酶基因发生多处杂合突变。白猴黑色素含量仅为正常猴的67%,同时Fe元素含量高于正常值。结论—白猴色素变化与酪氨酶基因的多态杂合突变有关,又与血液中Fe元素含量升高有关,但二者之间是否有联系,有何种联系却尚待进一步研究。
Objective In order to study the molecular mechanism of the pigment disorder of a novel white monkey, we detected several possibilities of the etiological factors. Methods The mutation of tyrosinase （TYR） gene was studied using DHPLC assay and DNA sequence. The melanin content and trace elements were assayed by spectrophotometer in A475 and anodic stripping voltammetry respectively. Results The results showed that there was no any change taken place in the blood, and found several heterozygotic mutations on the TYR gene. Meanwhile, the content of melanin was just 67 % of the normal monkey, and Fe was higher in this white monkey. Conclusion Pigment disorder of this white monkey has some relationships with the heterozygotic mutation polymorphism of TYR gene, moreover, relates to the Fe content going up in the blood. However, if there has any correlation between the mutations of TYR and Fe rising up, it needs further studying.
出处
《四川动物》
CSCD
北大核心
2006年第4期730-735,F0004,共7页
Sichuan Journal of Zoology
