摘要
目的:通过体内、体外实验探讨红景天提取物抗肿瘤作用。方法:实验于2004-03/2005-05在新疆医科大学细胞实验室、动物中心和分子生物学实验室完成。①体外抗肿瘤实验:应用四甲基偶氮唑盐法检测红景天提取物对泌尿生殖系统中4个肿瘤细胞株[人宫颈癌细胞株(Hela)、人乳腺癌细胞株(Bcap-37)、人卵巢癌细胞株(HO8910)、人膀胱癌细胞株(T24)]生长的抑制作用。细胞实验分红景天提取物0.1,0.01,0.001,0.0001g/mL4个浓度组、阴性对照组(含同一细胞密度的培养液)、溶剂对照组(75g/L甘油)。②体内抗肿瘤实验:取40只昆明小鼠随机数字表法分为4组:模型对照组、高剂量组(2000mg/kg)、中剂量组(1000mg/kg)、低剂量组(500mg/kg)。提取物对移植的S180腹水瘤小鼠存活的影响:每鼠腹腔注射腹水0.2mL。注射的第2天开始灌胃,各干预组给予不同剂量的相应提取物,模型对照组给予同体积的蒸馏水,干预时间直至小鼠死亡为止,记录小鼠死亡时间,根据公式计算生命延长率=(实验组平均存活时间/对照组平均存活时间-1)×100%。提取物对移植的S180实体瘤小鼠肿瘤的抑制作用:取已制备的接种混悬液0.1mL,注射于接种小鼠的左后肢内侧皮下。注射后的第2天开始灌胃,各干预组给予不同剂量的相应提取物,模型对照组给予同体积的蒸馏水,灌胃时间为每天16:00～18:00,干预时间为2周。每7d称量小鼠体质量1次,干预结束后第1天剥离肿瘤称重并记录,计算肿瘤生长抑制率=(阴性对照组平均瘤重-干预组平均数瘤重)/阴性对照组平均瘤重×100%。结果:纳入动物40只,均进入结果分析。①红景天提取物各浓度对人乳腺癌细胞株(Bcap-37)、人卵巢癌细胞株(HO8910)细胞的生长均有抑制作用[以浓度为0.01g/mL为例,红景天提取物组和阴性对照组分别为0.427±0.049,0.662±0.025;0.450±0.031,0.606±0.041,P<0.01]。红景天提取物浓度为0.001～0.1g/mL对人宫颈癌细胞株(Hela)、人膀胱癌细胞株(T24)细胞的生长有抑制作用[以浓度为0.01g/mL为例,红景天提取物组和阴性对照组分别为0.411±0.060,0.607±0.018;0.326±0.030,0.701±0.014,P<0.01],而浓度为0.0001g/mL无抑制作用。②红景天提取物高中低剂量组S180腹水瘤小鼠平均存活时间明显高于模型对照组[分别为(16.30±2.36),(14.30±2.67),(14.50±3.95),(8.90±2.60)d,P<0.01];高中低剂量组S180实体瘤小鼠平均瘤重明显低于模型对照组[分别为(1.67±0.80),(1.54±1.22),(1.75±0.24),(3.09±0.98)g,P<0.05]。结论:红景天提取物体外实验对4种肿瘤细胞(Hela,Bcap,T24,HO8910)有良好的抑瘤作用;体内实验明显抑制了S180实体瘤小鼠肿瘤的生长并延长了S180腹水瘤小鼠的生存时间。红景天提取物具有一定的抗肿瘤效果。
AIM： To study the anti-tumor effects of the extract from Rhodiola root in viva and in vitro.
METHODS： The experiment was conducted in the Cell Laboratory, Animal Center and Molecular Biological Laboratory of Xinjiang Medical University from March 2004 to April 2005, （1）Anti-tumor experiment in vitro： The inhibitory effects of the extract on the growth of four kinds of cultured human cancer cell lines （Hela, Bcap-37, HO8910 and T24） were evaluated by MTT assay. There were 4 groups： extracts group with concentration of 0.1, 0.01, 0.001, 0.000 1 g/mL, negative control group （containing nutrient solution at the same concentration to the cell）, and dissolvent control group （75 g/L glycerine）. （2）Anti-tumor experiment in viva： 40 Kunming mice were, divided into 4 groups： model group, high （2 000 mg/kg）, middle （1 000 mg/kg） and low （500 mg/kg） dose groups, Effect of the extract on survival of mice with implanted ascite sareome-180： The mice were intraperitoneally injected with 0.2 mL ascitic fluid. From the 2^nd day, the mice in different groups were intragastrically infused with different doses of corresponding extracts. The model control group was given the matching distilled water. All the administration was given until the mice died and the death time was recorded. The life-prolonged rate was calculated by formula： （mean survival time of the experiment group/mean survival time of control group-1）×100%. Effect of the extract on inhibition of mice with implanted solid sarcome-180：0.1 mL inoculated suspension was subcutaneously injected into the left hind limb of mice. From the 2^nd day, the mice in different groups were intragastrically infused with different doses of corresponding extracts and the model control group was given the matching distilled water from 16：00 to 18：00 everyday for 2 weeks, The body mass of the mice was weighed every 7 days. The tumor was removed aod weighed at the first day after intervention, and the tumor growth inhibitory rate was calculated： （mean weight of tumor of the negative control group-mean tumor weight of the treatment group）/mean weight of tumor of the negative control group×100%.
RESULTS： All the 40 mice were involved in the result analysis.（1）The extract at different doses had inhibitory effects on these human cancer cell lines of Bcap-37, HO8910 cancer cell lines [0.01 g/mL extract group and negative control group： （0.427±0.049, 0.662±0.025; 0.450±0.031, 0.606±0.041）, P 〈 0.01]; The extract group at dose of 0.001-0.1 g/mL had inhibitory effects on these human cancer cell linesof Hela and T24 [0.01 g/mL extract group and negative control group： （0.411±0.060, 0.607±0.018; 0.326±0.030, 0.701±0.014）, P 〈 0.01];but the extract with the dose of 0.000 1 g/mL had no inhibitory effect. （2）The mean survival time of mice with ascites sarcoma-180 in the extract groups at different doses was obviously higher than the control group [（16.30±2.36）, （14.30±2.67）, （14.50±3.95）, （8.90±2.60） days, P 〈 0.01]; the mean weight of tumor tissues of Ihe mice with solid sarcoma-180 in the extract groups was much less than the model control group [（1.67±0.80）, （1.54±1.22）, （1.75±0.24）, （3.09 ±0.98）, P 〈 0.05].
CONCLUSION： The extract from Rhodiola root has inhibitory effects on the growth of four kinds of cultured human cancer cell lines （Hela, Beap- 37, T24 and HO8910）, The extract possesses obviously anti-tumor effects on the solid sarcoma-180 and prolongs the survival time of mice with ascites sarcoma-180. The extract from Rhodiola root has anti-tumor effects.
出处
《中国临床康复》
CSCD
北大核心
2006年第43期142-144,F0003,共4页
Chinese Journal of Clinical Rehabilitation
