摘要
目的测定非小细胞肺癌MVC和间质中TAMs、MCs计数以及趋化因子IL-8、MCP-1、MIP-1mRNA的表达,探讨其间相互关系。方法用免疫组化ABC法测定40例非小细胞肺癌组织石蜡切片标本中MV和TAMs、MCs计数。以原位杂交法检测上述标本中IL-8、MCP-1、MIP-1mRNA的表达情况。分析MV、TAMs、MCs计数和趋化因子阳性表达之间的相互关系。结果40例非小细胞肺癌组织中MV、TAMs、MCs计数和IL-8、MCP-1、MIP-1阳性表达之间以及它们相互之间均存在着密切的正相关。结论非小细胞肺癌间质炎性细胞TAMs、MCs和趋化因子IL-8、MCP-1、MIP-1能够相互协同,共同促进肿瘤血管生成。
[Objectives] To measure tumor microvessel density, interstitial infiltrating TMAs, MCs counts, and the expressions of chemokines IL-8, MCP-1, MIP-1 mRNA, while to evaluate their correlation. [Methods] Immunohistochemical staining （ABC method ） was carried out to measure microvessel counts and interstitial infiltrating TAMs, MCs counts in 40 NSCLC tissues.In situ hybridization was used to measure expressions of chemokine IL-8, MCP-1, MIP-1 mRNA in those specimens. The correlation of MV, TAMs, MCs counts and IL-8, MCP-1, MIP-1 mRNA positive expressions was analysed. [Results] In 40 NSCLC tissues, Between MV, TAMs, MCs counts and IL-8, MCP-1, MIP-1 mRNA positive expressions shown mutually positive correlation. [Conclusions] NSCLC interstitial infiltrating inflammatory ccU TAMs, MCs and chemokine IL-8, MIP-1, MIP-1 can cooperatively promote tumor angiogenesis.
出处
《中国医学工程》
2006年第5期459-461,465,共4页
China Medical Engineering
关键词
非小细胞肺癌
癌间质炎性细胞
趋化因子
免疫组化
原位杂交
NSCLC
interstitial infiltrating inflammatory
chemokine
immunohistochemistry
In situ hybridization
