摘要
目的:研究凤尾草抑制前列腺上皮细胞增生的活性化学成分。方法:大孔树脂、凝胶、正相及反相硅胶柱层析方法用于化合物的分离;红外、紫外、核磁和质谱等方法用于鉴定化合物结构。化合物对大鼠前列腺上皮细胞的抑制活性用MTT方法测定。结果:从凤尾草的丙酮和20%乙醇提取物中,分离并鉴定了22个化合物,其中化合物木犀草素,棕榈酸和芹菜素4′-O-α-L-鼠李糖苷显示与爱普列特相比拟的大鼠前列腺上皮细胞增生体外抑制效应。结论:化合物ptero-sideP′(1)是一个新的C14的倍半萜苷;化合物木犀草素、棕榈酸和芹菜素4′-O-α-L-鼠李糖苷可能是凤尾草能抑制良性前列腺增生的主要活性成分。
AIM： To study the active chemical constituents of Pteris nudtifida against growth of rat prostatic epithelial ceils （PECs）. METHODS： Column chromatographies of macmpomns resin D-1400, Sephadex LH-20, Rp-18, silica gel were used for the isolation of compounds, and spectroscopic techniques （NMR, IR, UV and MS） were used for the structural identification. The inhibitory activities of compounds on rat PECs growth were evaluated by MTT method under serum-free conditions with or without growth factors added to the culture medium. RESULTS： 22 compounds were isolated from acetone and 20% ethanol extracts of the tided plant. Among them, luteolin, palmitic acid and apigenin 4＇-O-α-L-rhamnopyranoside showed close effect against growth of rat PECs in vitro with epristeride. CONCLUSION： Ptemside P＇ （1） was identified to be a new C14 sesquiterpene glycoside; luteolin, palmitic acid and apigenin 4＇- O-α-L-rhamnopyranoside may be the main active components of Pteris multifida against benign prostatic hypertrophy.
出处
《中国天然药物》
SCIE
CAS
CSCD
2006年第6期428-431,共4页
