摘要
目的:从光棘球海胆(Strongylocentrotusnudus)中分离纯化具有生物活性的海胆壳蛋白。方法:海胆壳先经丙酮脱脂,然后生理盐水提取、超滤、离心得海胆壳蛋白粗品。粗品经超滤、DEAE-SepharoseFastFlow及SephacrylS-400柱层析纯化得海胆壳蛋白SUP-1A。经聚丙烯酰胺凝胶电泳和高效液相色谱鉴定其纯度达90%以上。通过MTT法测定了SUP-1A的体外抗肿瘤活性。结果:从海胆壳中分离纯化得到单一蛋白组分的SUP-1A,MTT法表明SUP-1A对多种肿瘤细胞的增殖都有较强的抑制作用。结论:从海胆壳中分离纯化到一种蛋白组分SUP-1A,其具有较强的体外抗肿瘤活性。
AIM： To extract and purify a bioactive protein from the shell of Strongylocentrotus nudus, and to determine its purity and antitumor activity in vitro. METHODS： The shell was extracted with normal saline. By ultrafiltration, DEAE Sepharose Fast Flow anionexchange chromatography and Sephacryl S-400 gel filtration chromatography, the crude protein was purified. The purity of SUP-1A was proved by HPLC and polyacrylamide gel electrophoresis.The antitumor activity was assayed with MTT method. RESULTS： SUP-1A was a homogeneous protein. SUP-1A remarkably inhibited the tumor proliferation. CONCLUSION： The homogeneous protein SUP-1A shows significant antitumor activity in vitro.
出处
《中国天然药物》
SCIE
CAS
CSCD
2006年第6期464-467,共4页
关键词
海胆蛋白
分离纯化
抗肿瘤
Strongylocentrotus nudus protein
Isolation and purification
Antitumor activity
