摘要
目的:观察雌性大鼠切除卵巢后其下丘脑弓状核β-内啡肽免疫反应神经元和血清生长素、雌激素、降钙素及骨钙素水平的变化。方法:实验于2004-03/2005-01在广东医学院生理学实验室完成。①将12只健康雌性未孕产SD大鼠采用随机数字表法分为2组,每组6只。卵巢切除术组大鼠在麻醉状态下完全切除双侧卵巢,牢固结扎剩余的子宫部分;假手术组大鼠不切除卵巢,仅切除卵巢周围小块脂肪组织。术后处理完全相同。②采用左心室灌注固定方法取胫骨及脑。通过检测大鼠胫骨上段骨组织形态计量学的变化判断骨代谢发生的变化。③用无菌注射器通过右心室取血,离心后取上层血清,采用放射免疫方法检测血清上述激素水平的改变。④采用免疫组织化学方法观察下丘脑弓状核β-内啡肽免疫反应神经元的变化。结果:①两组大鼠胫骨上段松质骨组织形态计量参数的变化:与假手术组大鼠比较,卵巢切除组大鼠骨小梁面积百分数、骨小梁宽度和骨小梁数量均明显减小眼穴22.1±3.3雪%比(14.8±2.3)%,(41.2±8.8)比(30.8±3.9)μm,(5.5±0.8)比(4.3±0.7)个,P<0.05或0.01演,骨小梁分离度明显增大眼(164.9±12.3)比(250.2±33.2)μm,P<0.01演。②两组大鼠血清激素水平的变化:与假手术组大鼠比较,卵巢切除组大鼠血清雌激素、降钙素、生长素含量明显降低眼穴35.22±3.41雪比(6.29±1.82)ng/L,(48.41±8.85)比(28.21±6.28)ng/L,(6.59±1.49)比(4.45±0.88)μg/L演,骨钙素明显升高眼穴13.72±4.58雪比(23.89±4.4)μg/L。③下丘脑弓状核β-内啡肽神经元的改变:卵巢切除组大鼠下丘脑弓状核β-内啡肽免疫反应阳性神经元的数量明显减少。结论:弓状核β-内啡肽免疫反应阳性神经元数量的减少及生长素等钙调激素水平的改变,可能与骨质疏松的形成具有密切的关系。
AIM： To observe the changes in the β-endorphin immunoreactive neurons of hypothalamus arcuate nucleus, serum growth hormone, estrogen, calcitionin and bone gla protein levels in ovariectomized female rats.
METHODS： The experiment was conducted in the physiological laboratory of Guangdong Medical College between March 2004 and January 2005. ① Twelve healthy female SD rats without pregnancy or production were randomly divided into 2 groups with 6 rats in each group： ovariectomized group： The rats were given bilateral ovariectomy under anesthesia, and the left uterine parts were ligated firmly; sham-operation group： The rats were cut off the fat tissue around ovary but not overiectomy, The treatment after operation was the same. ② The shin bone and brain of the rats were removed by left ventricle perfusion and fixation method. The changes in bone metabolism were judged by measured the bone histomorphometry. ③ The blood from right ventricle was removed with sterile injector, and the upper serum was taken after centrifugalization, Radioimmunoassay was performed to detect the serum levels of growth hormone, estrogen, calcitonin and bone gla protein. ④ Immunohistochemistry was used to observe the changes in the β-endorphin immunoreactive neurons of hypothalamus arcuate nucleus.
RESULTS： ① Changes in bone histomorphometry of the two groups： Compared with the sham-operation group, the trabecular area percentage, trabeeular thickness and trabecular number of the ovariectomized group were significantly reduced [（22.1±3.3）% vs. （14.8±2.3）%, （41.2±8.8） vs. （30.8±3.9） μm, （5.5±0.8） vs. （4.3±0,7）, P 〈 0.05 or 0.01], while the trabecular separation was significantly increased [（164.9±12.3） vs. （250.2±33.2） μm, P 〈 0.01]. ② Changes in serum levels： Compared with the sham-operation group, the estrogen, calcitonin and growth hormone of the ovariectomized group were markedly decreased [（35.22±3.41） vs. （6.29±1.82） ng/L, （48.41±8.85） vs. （28.21±6.28） ng/L, （6.59±1.49） vs. （4.45±0.88） μg/L], while the bone gla protein was significandy increased [（13.72±4.58） vs. （23.89±4.4） μg/L]. ③ Changes in the number of β-endorphin immunoreactive neurons of hypothalamus areuate nucleus： The number of the ovariectomied group was significantly decreased.
CONCLUSION： The reduction of β-endorphin immunoreactive neurons of hypothalamus arcuate nucleus and changes in growth hormone and other calcium-regulating hormones may have close relation with the formation of osteoporosis.
出处
《中国临床康复》
CSCD
北大核心
2006年第46期135-137,I0004,共4页
Chinese Journal of Clinical Rehabilitation
