摘要
目的探讨实验性脊髓空洞前状态的演变过程及其指导意义。方法制作动物模型,术后1、3、7、14、21 d处死,应用干湿重法测定脊髓含水量,并应用光镜、电镜观察脊髓空洞前状态组织学和超微结构改变。结果Kaolin组动物于术后第1天脊髓含水量即有明显增加(68．35±0．70)％,第7天达到高峰(72．92±0．86)％,持续到第14天,至21 d时稍有缓解(70．03±0．77)％。组织学发现术后1～7 d上颈髓主要为细胞毒性水肿,无亚细胞结构损害;术后第14天出现以血管源性水肿为主的混合性水肿,亚细胞结构出现损害。第21天出现前角细胞数量减少,胶质细胞增生,髓鞘板层状结构断裂,呈脱髓鞘改变,甚至轴索破坏。结论脊髓空洞前状态是防治空洞形成最佳时期,及时逆转缺血水肿将有助于预防脊髓空洞的形成。
Objective To investigate the developing process of experimental presyrinx state and its clinical guiding implications. Methods The experimental models of presyrinx state in rabbits were established by intra-cisternal injection of kaolin. Spinal water content, histological and uhrastructural changes were observed by dry-wet measure, light and electronic microscopy on the postoperative day 1,3, 7,14 and 21 respectively. Results Water content assay showed that spinal cord edema in Kaolin group animals appeared at the 1st day [ （68.35±0.70）% ] after kaolin injection, was more prominent at 3rd day [ （72.70 ±0.88） % ], reached its peak at 7th day [ （72.92±0.86） % ], and declined slowly after 3 weeks [ （70.03 ±0.77） % ]. Histologically, cytotoxic edema without subccllular structure damage was observed in upper cervical cord at 1st to 7th day after operation. Until 14th day, mixing edema but mainly with vasogenic edema occurred, companied with subcellular structure damages. Eventuly, at 21st day the number of cells in posterior horn was decreased, and glial cells proliferated. The lesions got so worse as to present demyelination characterized by brisement and fragmentation of lamellar texture of myelin sheath, and even axis cylinder destroyed. Conclusion Presyrinx state is the optimal period to prevent spinal porosis and proper termination of ischemia and edema of spinsl cord in presyrinx period will be helpful to prevent occurrence of syringomyelia.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第12期1444-1446,I0019,共4页
Chinese Journal of Experimental Surgery
基金
河北省科技厅资助项目(002761100)
关键词
脊髓空洞
组织学
电镜
兔
Syringomyelia
Histology
Electronic microscopy
Rabbit
