摘要
新鲜猪精液经乙醇沉淀，丙酮清洗，得抑制素粗提物，再经ＳｅｐｈａｄｅｘＧ－１００凝胶过滤，收集蛋白质洗脱峰。采用小鼠ｈＣＧ卵巢增重抑制法（改良斯蒂尔曼—波莱生物测定法）对其进行生物活性测定，粗品１０００μｇ剂量组对性成熟前雌性小鼠卵巢增重的抑制作用极显著（Ｐ＜０．０１），第二峰下降部分２００μｇ组的抑制效果显著（Ｐ＜０．０２）。表明从猪精液中可提取到具有生物活性的抑制素制品。
Fresh semen was precipitated with ethanol and washed with acetone to obtain a crude extract of porcine semen inhibin(psIB), and then purified by Sephadex G-100 Gel filtration to collect protein elution surge.The bioassay with hCG treated immature female mice by Modified Steelman Pohley method showed that the crude extract(dose 1 000 μg group) and the latter half fraction of the second surge (dose 200 μg group) significantly decreased the ovarian weight of hCG treated animals (P<0.01 and P<0.02). It seems that the preparation extracted from porcine semen has a biological activity of inhibin.
出处
《新疆农业大学学报》
CAS
1996年第3期8-11,共4页
Journal of Xinjiang Agricultural University
基金
国家自然科学基金
