摘要
目的：建立参麦注射液中3种人参皂苷Rg1、Re和Rb1的含量测定方法。方法：高效液相色谱法，色谱柱为C18柱，以乙腈为流动相A，水为流动相B，进行梯度洗脱。检测波长为203nm。结果：人参皂苷Rg1、Re和Rb1的线性范围分剐为0．4810～4．810μg，0．4025～4．025μg和0．6005～6．005μg。平均回收率分别为99．88％（RSD为0．34％，n=6），99．69％（RSD为0．36％，n=6）和99．59％（RSD为0．43％，n=6）。结论：本法简单、快速，结果准确。
OBJECTIVE To establish an HPLC method of determination of ginsenoside Rg1 ,ginsenoside Re and ginsenoside Rb1 in Shenmai injection. METHODS C18 column was used with mobile phrase composed of A and B and the detection wavelength 203 nm. RESULTS The calibration curves were separately linear of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 between 0. 481 0 - 4. 810μg,0. 402 5 - 4. 025μg and 0. 600 5 - 6. 005 μg;The average recoveries of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 were 99. 88%（RSD = 0. 34%, n=6）, 99. 69% （RSD = 0. 36%, n = 6） and 99. 59%（RSD = 0. 43%, n=6）. CONCLUSION The method is simple, quick, reproducible and is suitable for the quality control of Shenmai injection.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2006年第12期1499-1500,共2页
Chinese Journal of Hospital Pharmacy
