摘要
采用Zeta电位方法测定胶原及其降解物的等电点，并与等电聚焦圆盘电泳的结果进行丁比较，考察了浓度、温度等因素对该测定方法的影响。实验结果表明Zeta电位法测得的等电点数值与等电聚焦法的结果相吻合，可以用该方法来测定胶原及其降解物的等电点。在25-40℃之间，随着温度的升高，Zeta电位法测得的等电点数值有稍微的升高，但其偏差均在0．1个pH值范围以内。Zeta电位法测定时，胶原蛋白溶液的浓度一般控制在0．2～0．5mg／mL左右，即使蛋白质量浓度在0．1mg／mL以下也不影响该方法对胶原等电点测量结果的准确性。
Zeta potential measurement was used for determining isoelectric points ot collagen and its degradations. The determination results were compared with those of isoelectrofocusing determinations. Several factors, such as concentration, temperature, were studied. The results showed that isoelectric point values which determined by Zeta potential method were coincident with that of isoelectric focusing determinations, so this method could be used to determine the isoelectric point of collagen and its degradations. between 25 ℃ and 40 ℃, the results would shift When the determination temperature increased to higher pH value, but the difference of the re- suits were less than 0. 1 pH. The concentration of collagen and its degradations should be controlled in 0. 2-0. 5mg/mL for this method, even which was less than 0. 1 mg/mL also could maintain good determination results.
出处
《皮革科学与工程》
CAS
2006年第6期40-43,共4页
Leather Science and Engineering
基金
四川大学青年基金资助项目(编号:200557)
关键词
等电点
胶原
明胶
ZETA电位
isoelectrie point
results. collagen
gelatin
zeta potential
