摘要
目的观察钙依赖性酪氨酸激酶2(Pyk2)在风湿性心脏病患者中的表达,与心肌纤维化指标的关系及临床意义。方法30例风湿性心脏病患者心肌组织(实验组)和6例正常心肌标本(对照组)行M asson染色测定心肌蓝色胶原灰度值,采用放射免疫法检测血清透明质酸(HA)、层粘连蛋白(LN)和Ⅳ型胶原(IV-C)含量,并通过免疫组织化学法和逆转录-聚合酶链反应(RT-PCR)法观察Pyk2蛋白和信使核糖核酸(mRNA)的表达,以及Pyk2表达与心肌纤维化指标的相关性。结果实验组血清HA,LN和IV-C均较对照组高(174.95±76.14μg/L vs.70.06±15.63μg/L,153.86±20.72μg/L vs.90.01±14.11μg/L,95.26±7.66μg/L vs.63.21±10.62μg/L;P=0.003,0.013,0.035)。实验组Pyk2蛋白吸光度和Pyk2 mRNA/磷酸甘油醛脱氢酶(GAPDH)明显高于对照组(0.325±0.032vs.0.106±0.013,0.870±0.085 vs.0.573±0.042;P=0.048,0.006)。心肌Pyk2蛋白表达与心肌纤维化指标HA、LN和IV-C含量呈明显正相关(r=0.611,0.743,0.829,P<0.01),心肌Pyk2 mRNA表达与LN,IV-C含量呈正相关(r=0.794,0.766,P<0.05)。结论Pyk2可能通过增加风湿性心脏病心肌胶原合成在风湿性心脏病心肌间质纤维化的发生、发展进程中发挥重要作用。
Objective To observe the expression of calcium-dependent proline-rich tyrosine kinase-2（Pyk2） in myocardium of rheumatic heart disease, the relationship between its role and cardiac fibrosis and clinical significance. Methods The blue myocardium collagen stain were analysed after Masson staining in 30 patients with rheumatic heart disease （RHD group） and 6 normal myocardium specimens （control group）. The contents of hyaluronic acid （HA）, laminin（LN） and type IV collagen（IV-C） were detected by radio-immunity method,and the expressions of Pyk2 protein and messenger ribonucleic acid（mRNA） were explored by immunohistochemistry methods and reverse transcriptase polymerase chain reaction （RT PCR）,then the correlations of these results were statistically analyzed. Results The contents of HA,LN and IV C in RHDgroup increased compared to control group（174.95±76.14μg/L vs. 70.06±15.63μg/L, 153. 86 ± 20. 72μg/L vs. 90.01±14. 11μg/L, 95. 26±7.66μg/L vs. 63. 21±10.62μg/L; P= 0.003, 0. 013, 0. 035）. The Pyk2 absorption and the ratio of Pyk2 mRNA/glyceraldehyde phosphate dehydrogenase （GAPDH） in RHD group were significantly higher than those in control group （0. 325 ± 0. 032 vs. 0.106±0.013, 0.870±0.085 vs. 0.573±0.042; P=0.048, 0.006）.There were positive correlativity between the expression of Pyk2 protein and HA, LN and IV-C （r=0. 611, 0. 743, 0. 829, P〈0. 01）, there were positive correlativity between the expression of Pyk2 mRNA and LN, IV-C （r=0. 794, 0. 766, P〈0.05）. Conclusion Pyk2 may play a key role in the proceeding of cardiac fibrosis in rheumatic heart disease by increasing collagen synthesis in myocardium.
出处
《中国胸心血管外科临床杂志》
CAS
2006年第6期414-417,共4页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
基金
第三军医大学青年基金资助项目(XG200530)
关键词
钙依赖性酪氨酸激酶
风湿性心脏病
心肌纤维化
Calcium-dependent proline-rich tyrosine kinase
Rheumatic heart disease
Cardiac fibrosis
