摘要
目的探讨利用自然衰老大鼠建立中风后遗症肾虚痰瘀病证模型的可行性。方法按中医肾虚痰瘀证标准从老龄鼠中进行筛选纳入老龄组,并设青龄组作对照。经检测部分老龄鼠相关指标、确定肾虚痰瘀证存在之后建立中风后遗症模型,于造模前后进行证候观察及相关指标检测并作反证治疗。结果老龄大鼠可见毛稀竖立、黯淡无泽、弓背、懒动、精神萎靡、反应迟钝、采食饮水减少、睡眠增多、阴囊皱缩、睾丸回升、舌质及尾部皮色暗或紫、眼球暗红等肾虚痰瘀证候,青龄大鼠未见上述证候;同时老龄鼠性功能、睾酮、红细胞SOD活性均低于青龄组(P<0.01),血浆MDA、血脂及血液流变学指标均高于青龄组(P<0.01)。造模后:老龄模型组出现了典型的偏瘫症状和肾虚痰瘀证候,经反证治疗明显改善;神经功能缺失评分:老龄模型组[(2.41±0.31)分]高于假手术组[(0.00±0.00)分](P<0.01),反证组[(1.50±0.33)分]低于老龄模型组[(2.41±0.31)分](P<0.05);红细胞SOD活性:老龄模型组低于老龄假手术组(P<0.01)和青龄模型组(P<0.05),反证组高于老龄模型组(P<0.01);血浆MDA:老龄模型组高于老龄假手术组(P<0.01)和青龄模型组(P<0.05),反证组低于老龄模型组(P<0.01);血清睾酮:反证组高于老龄模型组(P<0.01);血脂及血液流变学指标:反证组低于老龄模型组(P<0.05);脑组织SOD活性:老龄模型组低于老龄假手术组(P<0.01)和青龄模型组(P<0.05),反证组高于老龄模型组(P<0.01);脑组织MDA:老龄模型组高于老龄假手术组(P<0.01)和青龄模型组(P<0.05),反证组低于老龄模型组(P<0.01)。结论经按肾虚痰瘀证标准筛选的自然衰老大鼠基本具备肾虚痰瘀证的生物学特点,以此类大鼠建立的中风后遗症模型可作为中风后遗症肾虚痰瘀病证模型。
Objective To study biological feature of natural senile ＂shenxu-tanyu＂ rat model with stroke sequelae. Methods According with traditional chinese medicine standard of ＂shenxu-tanyu＂ syndrome, screening old rats to be the oht group and compared with the young group to set stroke sequelae rondel, taking syndrome observation and related index detection before and after model building with eounterevideaee therapy. Results Old rat shows characteristic of ＂shenxu-tanyu＂ syndrome： rare and erect hair, dark, bowed back, lazy, depressed, dullness, reduced diet and hydroposia, somnolence, serotum erenation, testic, le raise back, tongt, e and tail color dark or purple, dark red cyeball etc, no such features in young rat. Sexual funetion,Ts and RC SOD activity of ohl rat are lowe, r than the young group（ P 〈 0.01 ）, but blood plasma MDA, blond-fat and index of blood rheology are all higher than the young group（ P〈 0.01 ）. After model：typical hemiplegia and ＂shenxu-tanyu＂ syndrome is appeared in the oht group and improved obviously after eounterevidenee therapy. Nerve function score： tire old group higber than SO group （ P〈 0.01 ） , counterevidence group lower tban the oht group （ P 〈0.05）. RC SOD activity： the oht group is lower than the old SO group （ P〈0.01 ） and the young group （ P〈0.05 ）, the eounterevidenee group is higher than the old group （ P〈0.01 ）. Blood plasma MDA： the old group is higher than the old SO gronp（ P 〈 0.01 ） and the young group （ P 〈0.05 ）, the counterevidence group is lower than the old group （ P〈 0. 01 ）, Serum Ts： the counterevidenec group is higher that, the old group （ P〈 0. 01 ） ; Bloud-fat and blood rheology index： the counterevidence group is lower than the old group （ P〈 0. 05） ; Brain tissue SOD activity： the old group is lower than the old SO group （ P〈0. 01 ） and tire young group （ P〈0. 05）, the counterevidence group is higher than the old group （ P〈0. 01 ）. Brain tissue MDA： the old group is higher than SO grnup （ P 〈 0.01 ） and the young group （ P〈 0. 05 ） , tire counterevidence group is lower than the old grovp （ P 〈 0.01 ）. Conclusions According to standard screen, natural senile rat basically has native biological feature of ＂shenxutanyu＂ syndrome, the stroke sequelae model established with this kind of rat can be a natural ＂shenxutanyu＂ syndrome model with stroke sequelae.
出处
《中国行为医学科学》
CSCD
2006年第12期1072-1075,共4页
Chinese Journal of Behavioral Medical Science
基金
山东省中医药科技发展计划项目(2005-014)
