摘要
目的对变性聚丙烯酰胺凝胶（PAGE）中DNA银染及显色方法进行改良，以得到简便、快速、经济、有效的DNA银染显色方法。方法改良的方法省略丫预处理、固定、用试剂终止显色过程等步骤，并无需中间用水冲洗、漂洗。结果改良方法使PAGE中DNA银染显色全程时间缩短至15～20min，且效果较好。结论改良后的方法简捷有效，值得应用。
Objective To improve the silver-stain and development of DNA in metamorphic polyacrylamide gel in order to obtain an easy, rapid, economic and effective method. Methods The modified method dispensed with the steps including pre-treatment, fixation, development ending by agents, and washing,bleaching were not necessary during the process. Results The total time of DNA silver-stain and development in polyacrylamide gel in this modified method was shortened to 15 - 20 minutes, and the image quality was better. Conclusion The modified method is easy, rapid and effective.
出处
《现代临床医学生物工程学杂志》
CAS
2006年第4期371-373,共3页
Journal of Modern Clinical Medical Bioengineering
关键词
聚丙烯酰胺
凝胶
银染
显色
Polypropylene acrylamide
Gelatin
Silver stain
Development
