摘要
黑藻(Hydrillaverticillata)养殖水对铜绿微囊藻(Microcysticaeruginosa)的生长有明显抑制作用。同时,它显著降低铜绿微囊藻的光合速率;而使呼吸速率与超氧物歧化酶(SOD)活性先升高,后迅速下降;使细胞膜透性显著增大。电镜照片显示,黑藻养殖水对铜绿微囊藻的超微结构产生进行性的损伤,对质膜、类囊体片层及拟核区的破坏尤为显著。黑藻养殖水的乙醚提取物有显著抑制微囊藻生长的作用,且呈现明显的量效关系,证实黑藻对铜绿微囊藻的克生作用是通过向水中分泌克藻物质实现的。经GC-MS分析和初步鉴定,黑藻养殖水的乙醚提取物中含有邻苯二甲酸二异辛酯、邻苯二甲酸二丁酯、邻苯二甲酸丁酯2-甲基丙酯等物质。
This paper studied on the effect and mechanism that the growth of M. aerugonsa was markedly inhibited by the H. verticillata culture water （Fig. 1）. During treatment, the photosynthetic rate of M. aerugonsa declined （Fig.2）, while its respiratory rate and SOD activity increased firstly, then decreased as the treatment went on （Figs.2, 3）. Its membrane permeability also increased significantly （Fig.3）. TEM photographs showed that the ultrastructure of cell membrane, thylakoid lamella and pith nucleoid of M. aerugonsa were destroyed severely （Fig.4）. Inhibitory effects could be observed only when the extracts were extracted by ether. The more extracts from ether, the better inhibitory effect observed （Table 1）. It suggested that the inhibitory effects of H. verticillata on M. aeruginosa were through excreting substances into water. GC/MS analytic result showed that the ether extract mainly consisted of 1,2-benzenedicarboxylic acid diisooctyl ester, dibutyl phthalate, and 1,2- benzenedicarboxylic acid butyl 2-methylpropyl ester （Fig.5）.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2006年第6期672-678,共7页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金项目(No.30370083)
教育部科学技术研究重要项目(No.01043)资助。~~
关键词
黑藻养殖水
铜绿微囊藻
抑制效应
抑制物分离
H. verticillata culture water
M. aeruginosa
inhibitory effect
isolation of inhibitors
