摘要
目的:探讨6周耐力训练及补充多糖提取物对大鼠血清中3种细胞因子水平和PBMC分泌3种细胞因子能力的影响。方法:120只雄性Wistar大鼠随机分为6组:耐力训练+黄芪多糖组、耐力训练+牛膝多糖组、单纯耐力训练组、安静+黄芪多糖组、安静+牛膝多糖组、安静对照组。大鼠进行6周递增负荷游泳训练后,观察大鼠血清中IL-2、IL-4、IL-10水平的改变以及外周血单个核淋巴细胞(PBMC)分泌IL-2、IL-4、IL-10能力的变化。结果:长期耐力训练后可造成大鼠血清IL-下降、IL-4、IL-10升高,PBMC分泌IL-4能力增高,表明长期耐力训练后Th1和Th2两型细胞的功能失衡。6周耐力训练同时补充黄芪多糖和牛膝多糖提取物能防止大鼠血清IL-2的明显下降及IL-4、IL-10升高;防止大鼠PBMC分泌IL-4的明显增高结论:长期耐力训练能引起辅助T细胞(Th)两个亚型Th1和Th2两型细胞功能失衡,这可能是长期耐力训练引起运动性免疫低下的原因之一。牛膝多糖和黄芪多糖提取物对耐力训练大鼠Th1和Th2型细胞平衡有一定的调节作用。
Objective：Study of intensive endurance training and supplementation of herbal polysaccharide extracts on levels of 1I.-2, IL-4,IL-10 in serum of rats. Methods ：120 Wistar Bats were randomly divided into 6 groups： sedentary control group（n =20）, sedentary with supplementation of APS group （n = 20）, sedentary with supplementation of ABPS group （n = 20）, training group （n = 20）, training with supplementation of APS group（n =20） and training with supplementation of ABPS group（n =20）. After 4-week and 6-week swimming, II.-2, IL-4,IL-10 in serum of rats and supernatant of PBMC were tested through ELISA. Results：After 6 weeks training, （ 1 ） In training group, the level of IL-2 in serum was lower than that in sedentary control group（ P 〈 0. 05 ）. On the contrary, the levels of IL-4, IL-10 in serum were increased evidently （ P 〈 0. 05）, while the level of IL-4 in the supernatant of PBMC was increased. （2） The changes of IL-2, IL-4 and IL-10 were inhibited by supplementation with ABPS or APS. Conclusion：The results indicate that supplementation with ABPS or APS may help regulate the balance of Thl and Th2 through modulating the levels of IL-2 , II,- 4 and IL-10 in rats during strenuous training.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第12期1119-1123,共5页
Chinese Journal of Immunology
基金
科学技术部国家科技攻关计划奥运科技专项(2002BA904B16)资助
