摘要
利用Vero传代细胞从病死水貂肝脏中分离获得1株病毒。该毒株可使培养细胞出现明显的细胞病变(CPE)。以提取病毒感染细胞总RNA为模板进行反转录聚合酶链式反应(RT-PCR),结果扩增出的基因片段大小与预期值相符。根据病毒的致细胞病变特征和RT-PCR鉴定结果,确证该毒株为犬瘟热病毒。
A mink-originated strain of canine distemper virus was isolated in Vero cell from a mink liver. CDVSJ strain exhibited a typical vacuous degeneration. A fragment of 843 bp was amplified from Vero cell incubated with the isolated virus strain by reverse transcriptase polymerase chain reaction（RT-PCR） . From the results described above, it can be concluded that the strain is a new strain of mink-originated CDV.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2006年第6期674-677,共4页
Journal of Jilin Agricultural University
基金
国家"948"项目(2001-364)
关键词
水貂
犬瘟热病毒
分离鉴定
RT-PCR
mink
canine distemper vims
isolation and identification
RT - PCR
