摘要
本文研制了用生物倒置显微镜和以微通道板为核心的超高灵敏度光电成像系统组成的光子计数显微成像系统，此系统具有高空间放大倍数（１６０×）和极高的光子放大倍数（１０７），可探测到大小为μｍ量级，发光强度为１００光子／秒的发光图像。应用此系统拍摄到了单个明亮发光杆菌（Ｐｈｏｔｏｂａｃｔｅｒｉｕｍｐｈｏｓｐｈｏｒｅｕｍ）的发光图像，图像显示明亮发光杆菌的大小为２．４×０．６０（μｍ）２，单个细菌的发光强度在１００光子／秒量级。上述实验为明亮杆菌在环境保护中的应用提供了基本参数，也为进一步从细胞水平上研究发光细菌提供了可能。
A photon counting micro-imaging system consisted of an invert biological microscope and an ultra-sensitive photo-electronic imaging part has for developed. It has high spactial magnification (160×) and ultra-high photon magnification(107), so it can detect illuminate image in μp of size and 100 photons persecond of illuminate intensity. Using this imaging system. we have obtained illuminate illuminate image of a single Photobacterium phosphoreum which shows that its size is about 2.4×0.6(μm)2 and the illuminate intensity is in the order of 100 photons persecond. The experiment can provide fundamental parameters for application of Photobacterium phosphoreum in environmental protecion, and it also give us a possible method for further research in cell's level.
出处
《生物物理学报》
CAS
CSCD
北大核心
1996年第2期326-330,共5页
Acta Biophysica Sinica
基金
国家自然科学基金
关键词
明亮杆菌
发光细菌
发光图像
光子计数成像
Photon counting imaging
Photobacterium phosphoreum Luminescent bacteria
Illuminate image
