摘要
三丁基锡（ＴＢＴ）是人红细胞膜上Ｎａ＾＋，Ｋ＾＋－ＡＴＰａｓｅ的一种抑制剂。该化合物对Ｎａ＾＋，Ｋ＾＋－ＡＴＰａｓｅ有很强的抑制能力。在正常的反应体系中，ＴＢＴ浓度仅为１０μｍｏｌ／Ｌ时，该酶的活性全部丧失；其抑制Ｎａ＾＋，Ｋ＾＋－ＡＴＰａｓｅ的ＩＣ５０值为２．２μｍｏｌ／Ｌ．Ｋ＾＋能增强ＴＢＴ的这种抑制作用，ＴＢＴ为Ｋ＾＋的反竞争性抑制剂。
TBT is a tin containing organic compound (tributyltin). The effect of TBT on Na￣+,K￣+ -ATPase activrty of human erythrocyte membrane was studied.It was found that TBT could inhibit Na￣+, K￣+ -ATPase activrty, the potency was very significant (IC_(50)= 2. 2 μmol/L).An amount of only 10 μmol/L TBT could inactivate approximateiy the intact enzyme activity.K￣+ could enhance the inhibition potency of TBT and TBT was the noncompetitive inhibitor of K￣+.
出处
《生物化学杂志》
CAS
CSCD
1996年第3期313-316,共4页
基金
国家自然科学基金
