摘要
目的采用产肠毒素大肠杆菌(ETEC)K88标准菌株,研究其不同浓度的菌毛及全菌两种抗原对蛋鸡的免疫原性,并确定最佳的抗原浓度。方法用SDS-PAGE鉴定菌毛蛋白的纯度,通过间接ELISA法检测特异性卵黄抗体(IgY)的效价。结果菌毛蛋白对蛋鸡的免疫原性优于全菌;纯化的菌毛蛋白对蛋鸡的免疫原性优于粗菌毛蛋白,其诱导的抗体效价最高可达1∶480000,初免84天后未见有明显的下降趋势;菌毛蛋白最佳浓度为2mg/ml,全菌最佳浓度为1010cfu/ml。结论本研究为制备高效价特异性IgY抗体奠定了基础。
Objective：Immunogenicity on laying hens was investigated following immunized with different antigen preparations. The antigen preparations used for the comparative studies were crude fimbriae, purified fimbriae and formalin-killed bacteria. Methods： The purity was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE）. The antibody titer was measured by ELISA using purified fimbriae as an antigen. Results ：The antibody titer was much greater when the fimbriae was used as an antigen （480 000） compared with that of antibodies with the formalin-killed bacteria（ 320 000）. Moreover the purified fimbriae dramatically increased the titer. The optimal concentration of purified fimbriae and formalin-killed bacteria was found to be 2 mg/ml and 10^10 cfu/ml respectively, which exclited a maximal and stable antibody titer. Conclusion ：The study can provide a novel alternative approach to obtain specific high titer IgY.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第11期1014-1017,共4页
Chinese Journal of Immunology
基金
国家自然科学基金(30371053)
国家杰出青年科学基金(30125034)
大连市科技计划项目(2003B3NS024)
振兴东北老工业基地项目(发改高技[2004]2057号)资助
