摘要
目的探讨环氧合酶2(COX-2)、前列腺素15-羟基脱氢酶(15-PGDH)在胎盘和胎膜组织中的表达变化及其与早产发生的关系。方法采用免疫组化二步法,测定14例早产产妇(早产组)、18例足月临产产妇(足月临产组)、17例足月未临产产妇(对照组)的胎盘和胎膜组织中COX-2与15-PGDH的定位与表达水平(以阳性百分数计分和细胞染色计分相加表示)。结果(1)COX-2在3组产妇的羊膜上皮细胞、绒毛膜细胞和蜕膜细胞的胞质中均有表达。COX-2在早产组胎膜和胎盘组织羊膜上皮细胞中的表达水平分别为(4·6±1·2)、(4·7±0·9)分,在足月临产组分别为(3·2±1·0)、(3·6±1·0)分,在对照组分别为(2·2±0·6)、(2·5±0·9)分。早产组及足月临产组明显高于对照组,两两比较,差异均有统计学意义(P<0·01);早产组与足月临产组比较,差异也有统计学意义(P<0·01)。3组间胎膜羊膜上皮细胞与胎盘羊膜上皮细胞中的COX-2表达水平相互比较,差异均无统计学意义(P>0·05)。(2)COX-2在早产组胎盘绒毛膜细胞中的表达水平为(4·9±1·0)分,在足月临产组为(3·9±1·2)分,在对照组为(2·3±0·7)分,早产组及足月临产组明显高于对照组,两两比较,差异均有统计学意义(P<0·01);早产组与足月临产组比较,差异也有统计学意义(P<0·01)。而3组胎膜绒毛膜组织中COX-2表达水平比较,差异无统计学意义(P>0·05);在3组胎盘蜕膜细胞中的表达水平比较,差异也无统计学意义(P>0·05)。(3)15-PGDH在3组产妇羊膜上皮细胞、绒毛膜细胞和蜕膜细胞的胞质中均有表达,3组胎膜及胎盘组织的羊膜上皮细胞中15-PGDH表达水平比较,差异无统计学意义(P>0·05);而在胎膜及胎盘组织的绒毛膜细胞中表达水平,早产组分别为(1·5±0·6)、(2·3±0·8)分,足月临产组分别为(2·6±0·8)、(3·0±0·7)分,对照组分别为(4·4±1·1)、(4·1±1·2)分,早产组及足月临产组明显低于对照组,两者比较,差异有统计学意义(P<0·01);早产组与足月临产组比较,差异也有统计学意义(P<0·05)。在胎盘组织蜕膜细胞中的表达水平,早产组为(2·1±0·7)分,足月临产组为(2·8±0·8)分,对照组为(4·5±1·0)分,早产组及足月临产组明显低于对照组,两者比较,差异有统计学意义(P<0·01);早产组与足月临产组比较,差异也有统计学意义(P<0·05)。结论COX-2在羊膜上皮细胞中的高表达和15-PGDH在绒毛膜、蜕膜细胞中的低表达,与早产的分娩发动有关。二者在早产的发生中起一定作用。
Objective To investigate the effect of cyclooxygenase 2 （COX-2） and 15-prostaglandin dehydrogenase （15-PGDH） on preterm labor. Methods The expressions and localizations of COX-2 and 15-PGDH in placentas and fetal membranes were examined by immunohistochemical （IH） two-step assay （ IH scores were expressed as the sum of the percentage of immunoreactivity and the stained degree of cells）, respectively. The samples were obtained from 14 preterm delivery （ PL）, 18 term in labor（TL） and 17 term not in labor （ control group ）. Results （ 1 ） The immunoreactivity of COX-2 was found in the amniotic epithelial cells, chorion cells and decidual cells. IH scores of COX-2 in amniotic epithelium of placenta or fetal membranes in PL were 4.6 ± 1.2, 4.7 ±0.9, respectively; in TL were 3.2 ± 1.0, 3.6 ± 1.0, respectively, and in control group were 2. 2 ± 0. 6, 2. 5 ± 0. 9, respectively. IH scores in PL and TL were obviously higher than those in control group （ P 〈0. 01 ）. It was higher in PL than in TL （ P 〈0. 01 ）. The expression of COX-2 was not significantly different between amnion of placenta and amnion of fetal membranes among the three groups （P 〉0. 05）. （2） IH scores of COX-2 in chorion of placenta in PL were 4.9±1.0, in TL were 3.9 ±1.2 and in control group were 2.3 ±0.7. IH scores in PL and TL were obviously higher than those in control group （ P 〈 0. 01 ）, and it was higher in PL than in TL（ P 〈 0. 01 ）. The expression of COX-2 was not significantly different among the three groups in chorion of fetal membranes （ P 〉 0.05 ） and it was not significantly different among the three groups in decidual cell of placenta （ P 〉 0. 05 ） , either. （ 3 ） The immunoreactivity of 15-PGDH was found in the amniotic epithelial cells, chorion cells and decidual cells. The expression of 15-PGDH was not significantly different among the three groups in amnion of placenta or fetal membranes（ P 〉 0. 05 ）. IH scores of 15-PGDH in chorion of placenta and fetal membranes in PL were 1.5 ± 0. 6,2. 3 ± 0. 8, respectively, in TL were 2. 6 ± 0. 8,3.0 ± 0. 7, respectively, and in control group were 4. 4 ± 1.1, 4. 1 ± 1.2, respectively. IH scores in PL and TL were obviously lower than those in control group（ P 〈 0. 01 ） , and it was lower in PL than in TL（ P 〈 0. 05 ）. IH scores of 15- PGDH in decidua of placenta in PL were 2. 1 ± 0. 7, in TL were 2. 8 ± 0. 8 and in control group were 4. 5 ± 1.0. IH scores in PL and TL were obviously lower than those in control group （ P 〈 0. 01 ） ; it was lower in PL than in TL（ P 〈 0. 05 ）. Conehtsions The higher expression of COX-2 in amniotic epithelium and the lower expression of 15-PGDH in chorion and decidual cell are relevant with the onset of premature labor. They play a role in the pathogenesis of premature labor.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2006年第12期793-798,共6页
Chinese Journal of Obstetrics and Gynecology
关键词
前列腺素内过氧化物合酶
羟前列腺素脱氢酶类
分娩
早产
Prostaglandin-endoperoxide synthase
Hydroxyprostaglandin dehydrogenases
Labor, premature
