摘要
目的探讨女性压力性尿失禁(SUI)患者阴道壁结缔组织中基质金属蛋白酶(MMP)9及其组织抑制物(TIMP)1的mRNA表达变化及其与SUI发病的关系。方法收集24例有完整随访资料的女性SUI患者的阴道壁结缔组织标本作为研究组,根据年龄不同分为>60岁和≤60岁两类,每组12例;另选7例非SUI妇女作为对照组,应用RT-PCR法检测MMP-9和TIMP-1mRNA表达量。以MMP-9、TIMP-1与内参照3-磷酸甘油醛脱氢酶(GAPDH)基因的扫描灰度,计算曲线下峰面积作为PCR产物含量。结果(1)研究组中>60岁和≤60岁者MMP-9mRNA的表达量分别为0·56±0·20和0·56±0·19,均高于对照组的0·37±0·18,分别比较,差异均有统计学意义(P<0·05);>60岁和≤60岁者MMP-9mRNA的表达量比较,差异无统计学意义(P>0·05)。(2)研究组中>60岁和≤60岁者TIMP-1mRNA的表达量分别为0·23±0·11和0·31±0·12,均低于对照组的0·41±0·13,分别比较,差异均有统计学意义(P<0·05),TIMP-1mRNA的表达量在>60岁和≤60岁者间比较,差异也有统计学意义(P<0·05)。(3)MMP-9/TIMP-1比值,研究组中>60岁和≤60岁者及对照组分别为2·49±1·82、1·82±1·58、0·90±1·38,分别比较,差异均有统计学意义(P<0·05)。结论SUI患者MMP-9的高表达和TIMP-1的低表达,导致MMP/TIMP比值升高,年龄越大,MMP/TIMP比值升高越显著;MMP与TIMP比例失衡,在SUI的发病过程中可能起重要作用。
Objective To study semi-quantitatively mRNA expression of matrix metalloproteinase-9 （MMP-9） and its inhibitor, tissue inhibitor of matrix metalloproteinase-1 （ TIMP-1 ）, in vaginal wall connective tissue in women with stress urinary incontinence （SUI） compared to continent controls, and to explore the relationship between MMP-9, TIMP-1 and SUI. Methods Vaginal wall tissues were obtained from 24 women with SUI who were followed-up（ 12 cases are 〉60 years old and 12 cases ≤60 years old）. Seven patients undergoing total hysterectomy for carcinoma in situ of cervix without urinary incontinence served as control group. RNA was extracted and quantified. Semi-quantitative competitive reverse transcription was carried out with oligo-nucleotide primers to quantify MMP-9 and TIMP-1 mRNA expression. Results We used GeneSnap to analyze the data. MMP-9 in three groups （ 〉 60, ≤60 years and control） was 0. 56 ± 0. 20,0. 56 ± 0. 19,0. 37 ± 0. 18, significantly decreased（ P 〈 0. 05 ）. There was no difference between 〉60 and ≤60 year age groups（P 〉0.05）. TIMP-1 in three groups was 0.23 ±0. 11,0.31 ± 0. 12,0. 41 ± 0. 13, significantly increased（ P 〈 0. 05 ）. There was a great difference between 〉 60 and ≤ 60 year age groups in TIMP expression（ P 〉 0. 05 ）. The ratio of MMP-9/TIMP-1 in 〉 60, ≤60 year age groups and control group was 2. 49 ± 1.82, 1.82 ± 1.58,0.90 ± 1.38, significantly decreased （ P 〈 0. 05 ）. Conclusion Stress urinary incontinent women demonstrate a significant increase in MMP-9 mRNA expression and significant decrease in TIMP-1 mRNA expression. In SUI patients, proportion of MMP-9 and TIMP- 1 was overbalanced. Both these findings are consistent with increased collagen breakdown and may play an important role.in the onset and development of SUI.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2006年第12期810-813,共4页
Chinese Journal of Obstetrics and Gynecology
基金
福建省科技攻关重点基金资助项目(2003Y016)
关键词
尿失禁
压力性
阴道
结缔组织
明胶酶B
金属蛋白酶1组织抑制剂
Urinary incontinence stress
Vagina
Connective, tissue
Gelatinase B
Tissue-inhibitor of metalloproteinase-1
